Air bubbles in my agar plates
bob
have little air bubbles/pockets embedded in my agar. I beleive some
gas is being release by the agar and trapped in the agar. The media I
use is LB. I don't know if it was Lennox broth or Luriel broth. LB
is LB to me. My bacterial still grows but the air bubbles makes things
a little harder to see and they also don't look very nice.
This occurs occasionally. What gas is being released and what can I
do to fix it?
Michael Witty
. . . happened to me intermittantly as well. Don't know what caused it.
Don't know what made it stop. Yes, it is annoying! Regards, Mike.
Subhash Kulkarni
that, just keep the molten media calm and then pour with agitation, that
should do the trick, no gas is being LIBERATED
Wolfgang Schechinger
As long as it's just LB, boil it *gently* for a minute to release all air
trapped inside. Let cool down and pour your plates or use 30 ml with a
pipet to cast 2 or 3 10 cm dishes.
Wo
At 15:30 21.04.2003 +0200, Subhash Kulkarni wrote:
>you are shaking the molten media too much before u plate it, avoid doing
>that, just keep the molten media calm and then pour with agitation, that
>should do the trick, no gas is being LIBERATED
>
>Michael Witty wrote:
---
Duncan Clark
<51c3a5d3.0304...@posting.google.com> on Sun, 20 Apr 2003,
bob <uts...@hotmail.com> penned the following literary masterpiece:
>what can I
>do to fix it?
Lightly (and I do mean lightly) flame the plate surface after pouring,
with a bunsen. You will never see bubbles again. Have done it for years.
Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
Duncan Clark
GeneSys Ltd.
taskan4
> with a bunsen. You will never see bubbles again. Have done it for
years.
>
> Duncan
I guess this is intended to get rid of "surface" bubbles that form when
pouring. The original message probably referred to bubbles at the bottom
of the plates (under the agar) that form after incubating at 37ºC. It
also happens to me if I dry the plates for too long before using them.
---
Duncan Clark
taskan4 <tas...@yahoo.es> penned the following literary masterpiece:
Ah, I didn't read it that way :-(
S.Ballal
One likely possibility is that the media is poured when it is very
close to solidification temp. Normally bubbles formed due to shaking
hot media generally come up after pouring and then you can "flame"
them. But when the media is getting cold, it it still viscous to flow
but guess solidifies soon trapping bubbles.
The bubbles at the bottom of the old plates come due to dehydration
and are formed as the media starts getting detached from the plate due
to storage in inverted condition.
Apart from this I also see no other reason why such a thing should
happen.
S.Ballal
Indus Biotherapeutics Ltd.
Ahmedabad, India
Duncan Clark <junk@[127.0.0.1]> wrote in message news:<PEgHyiDOrUp+EAY2@[127.0.0.1]>...
bob
> Historians believe that in newspost
> <51c3a5d3.0304...@posting.google.com> on Sun, 20 Apr 2003,
> bob <uts...@hotmail.com> penned the following literary masterpiece:
> >what can I
> >do to fix it?
>
> Lightly (and I do mean lightly) flame the plate surface after pouring,
> with a bunsen. You will never see bubbles again. Have done it for years.
>
> Duncan
I already do that. I'm not speaking of surface bubbles that occur
during casting. I'm reffering to bubbles that form after I place them
in 27 degress celcius. These bubbles are embedded in the agar.
Tom Tomzynski
Are your plates cold when you incubate them?
This happens to me sometimes when plates are incubated
still cold from the refrigerator. Try letting them
warm up to room temperature before incubation.
Spreading a single layer of plates on the bench works well.
Gases are more soluble in the cold, so when the plates
are warmed up quickly, the gas comes out of solution quickly.
Rafael Maldonado
bob wrote:
> I already do that. I'm not speaking of surface bubbles that occur
> during casting. I'm reffering to bubbles that form after I place them
> in 27 degress celcius. These bubbles are embedded in the agar.
Are those bubbles embedded in the agar, or between the agar and the
plastic plate? Sometimes I have got bubbles between the agar and the
plastic, due to changes in temperature, when plates are incubated at 37
coming directly from the fridge...
--
Rafael Maldonado, Ph. D.
Division of Genetics
University of Alicante
Spain
taskan4
that happens during long periods of storage at 4ºC, even in plates
sealed with parafilm. Any ideas?
---
Subhash Kulkarni
then came the generation of using glass plates and flaming the lid, then
came the generation of simple pouring the water out in the sterile
chamber, and the search for a better alternative continues, i guess the
best wayout is to put a small dehumidifier or a dessicator in ur
chambers, it works fine for me....
Nick Theodorakis
>A friend of mine wants to avoid the accumulation of water in agar plates
>that happens during long periods of storage at 4=BAC, even in plates
>sealed with parafilm. Any ideas?
>
First, if you are not already doing so now, let then sit out overnight
after pouring them to allow them to dry a bit before storage. Second,
store them upside down (agar on top) so that if you do get water, it
stays on the plastic lid, and you can just shake it off.
Nick
--
Nick Theodorakis
nicholas_t...@urmc.rochester.edu
