C. elegans protein extraction.

[Stuart Rison]

Dear all,

A collegue of mine is looking for a quick and easy way of extracting C.
elegans proteins into buffer.

He claims that, in his previous attempt, C. elegans worms survived polytron
homogenisation and sonication in a triton X-100 buffer (i.e. there were
still some worms intact and ALIVE post-treatment).

Is there a more succesful technique?

Cheers,

Stuart.

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| Stuart Rison | Ludwig Institute for Cancer Research |
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- And there's a NO_JUNK bit to remove in my return address -

[R. John Lye]

Stuart Rison wrote:
> He claims that, in his previous attempt, C. elegans worms survived polytron
> homogenisation and sonication in a triton X-100 buffer (i.e. there were
> still some worms intact and ALIVE post-treatment).

Yeah, they're tough little buggers.

> Is there a more succesful technique?

I used a French press set at 5,000 psi internal pressure.

good luck,
--
John Lye

e-mail to: rj...@Virginia.EDU
homepage: http://avery.med.virginia.edu/~rjl6n/homepage.htm