Differences between Detergents
Byung-Hoon Kim
could anybody tell me the functional difference bwtween Triton X-100,
Tween20 and NP-40?
Thanks in advance
Byung-hoon Kim
Cornelius Krasel
> could anybody tell me the functional difference bwtween Triton X-100,
> Tween20 and NP-40?
What do you mean with "functional difference"?
Examples for differences: Triton X-100 and NP-40 are both aromatic
detergents and therefore quite unusable if you want to measure UV
absorption of the solution. Tween-20 is not aromatic.
CMCs: NP-40: 0.05-0.3 mM
Tx100: 0.2-0.9 mM
Tw-20: 0.059 mM
(from the Calbiochem detergent brochure). CMCs are important if you want
to dialyse your solution or concentrate it via membrane filtration.
The detergent that you will finally use has probably to be found out by
trial and error - there is no generally accepted rule which detergent to
try, as far as I know.
--Cornelius.
--
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany email: pha...@rzbox.uni-wuerzburg.de SP4 */
/* "Science is the game we play with God to find out what His rules are." */
Byung-Hoon Kim
Cornelius Krasel schrieb:
Oh, I'm sorry.........and...... thanks for your comment.
Actually I meant the functional difference in biochemical system.
For instance, protein-protein interaction, solubilization of unsoluble complex,
etc......
Byung-hoon
DOCT...@aol.com
the Calbiochem detergent booklet.
---
Nick Theodorakis
Could somebody tell me exactly what the difference is? I've been trying
to find out for years, but I get either, "they are the same," or, "they
are close, but not exactly the same." So far all I can tell is that
they are trademarked by different companies. Are they really the same
detergent but with different syntheses and different contaminants?
Note, I'm assuming everyone here means Nonidet P-40, not what Sigma
calls "NP-40."
Nick
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Cornelius Krasel
> Cornelius Krasel schrieb:
>> Byung-Hoon Kim <byung-h...@uni-tuebingen.de> wrote:
>> > could anybody tell me the functional difference bwtween Triton X-100,
>> > Tween20 and NP-40?
>>
>> What do you mean with "functional difference"?
[some examples snipped]
> Actually I meant the functional difference in biochemical system.
> For instance, protein-protein interaction, solubilization of unsoluble
> complex, etc......
I think Tween-20 is generally considered a "milder" detergent than
Tx-100 and NP-40 which seem to be fairly similar, if one can trust
the Calbiochem brochure (which you should really get - it's free and
one of the better sources of information about a lot of detergents).
Even milder are sugar derivatives, like dodecylmaltoside or octylglucoside.
Another very popular detergent for initial solubilization studies is
digitonin which is very kind to proteins as well, but rather impure
and quite damaging to all kinds of column material.
NP-40 and Tween-20 are quite often used in coimmunoprecipitation
experiments; therefore one would expect that they don't affect
protein-protein interactions too much. Again, if one of them
doesn't work for you, you can still try the other one.
Hope that helps,
Cornelius Krasel
> In article <0.f57e5ea...@aol.com>, DOCT...@aol.com wrote:
>> TritonX100 and NP40 are very similar but not identical. This is
>> not clear in the Calbiochem detergent booklet.
>
> to find out for years, but I get either, "they are the same," or, "they
> are close, but not exactly the same." So far all I can tell is that
> they are trademarked by different companies. Are they really the same
> detergent but with different syntheses and different contaminants?
>
> Note, I'm assuming everyone here means Nonidet P-40, not what Sigma
> calls "NP-40."
Sigma says:
"NP-40": nonylphenoxy polyethoxy ethanol
Nonidet P40: octylphenoxy polyethoxy ethanol (sold by Sigma as Igepal
CA-630; according to Sigma, Nonidet P40 is no longer manufactured)
Triton X-100: t-Octylphenoxy polyethoxy ethanol
"Nonidet P40" may have another isomer of octyl group at the phenol ring
than Triton X-100, where the group looks, according to Sigma, like
this:
CH3
|
(CH3)3-CH2-C-
|
CH3
No structure is given for "Nonidet P40", though.
The Sigma catalogue, in unusual verbosity, also notes that Triton X-100
is not a chemically defined substance. The number of ethoxy groups,
including the terminal ethanol, is given as approx. 9.5.
hzhen@freeuk
Byung-Hoon Kim wrote:
> Hi everyone,
>
> could anybody tell me the functional difference bwtween Triton X-100,
> Tween20 and NP-40?
>
> Thanks in advance
>
> Byung-hoon Kim
I don't know if it will give you the answer you need, but have a look in
the following references:
Detergents: An Overview, Neugebauer, J. M. (1990) Methods Enzymol. 182,
239-253.
Properties of Detergents, Helenius, A., McCaslin, D. R., Fries, E., and
Tanford, C. (1979) Methods Enzymol. 56, 734-749.