FREE Software Tool on NEW qPCR Homepage !!!
genequantification
Main focus of GENE QUANTIFICATION web page is to describe
and summarize all technical aspects involved in quantitative
gene expression analysis using real-time (RT-) PCR.
( specificity, sensitivity, reproducibility, intra- and inter-assay
variations, etc.)
The GENE QUANTIFICATION web page illustrates
the usefulness of a reliable quantification strategy,
and the difference between absolute vs. relative quantification
assays in real-time (RT-) PCR.
RT-PCR is the technique of choice for analysing mRNA in extremely low
abundance.
Real-time RT-PCR using SYBR Green I detection combines the ease
and necessary exactness to be able to produce reliable as well as
rapid results.
To obtain high accuracy and reliability in RT and real-time PCR highly
defined
quantification strategies and methods are needed.
http://www.wzw.tum.de/gene-quantification/ content:
- Real-time (RT-) PCR and optimisation !!!
- Available real-time PCR platforms
- Description of quantification strategies in real-time (RT-) PCR
- Description of absolute quantification and relative
quantification
- REST © ( Relative Expression Software Tool © )
- Housekeeping Gene problematic in real-time PCR ( under
construction !! )
- Verification of array results via kinetic RT-PCR ( under
construction !! )
- Comparison of real-time RT-PCR versus competitive RT-PCR
- Download of software tools (e.g. REST © )
- Summary of relevant literature
- Summary of relevant links
for further information contact Dr. Michael W. Pfaffl at