constitutive promoters needed

[Holger Merker]

Dear all,

I am looking for constitutive prokaryotic promoters (especially for
E.coli, B.subtilis, Pseudomons fluorescens and putida, Acinetobacter)
differing in their transcriptional activity except for the lac-promoter
or the beta-lactamase promoter. I only found this webpage
(http://margalit.huji.ac.il/) but I would like to know whether the
specific promoter is a weak or strong promoter (beside the fact that it
is constitutive).
So, does anyone of you know a database containing such information? or
could you suggest me some further constitutive promoters with regard to
their transcriptional activity?

Any suggestion/hint/comment is welcome. Thx a lot for your help.

All the best, Holger

--
Dipl.Biol. Holger Merker

Max-Planck-Institut f�r Chemische �kologie
Abteilung f�r Bioorganische Chemie (Prof. Boland)

Hans-Kn�ll-Strasse 8
07745 Jena
Germany

Tel.: +49-(0)3641-571212

[Venken, Koen J. T.]

I do not know of a database but you may want to look at (for E.coli at least):
-Escherichia coli strains with promoter libraries constructed by Red/ET recombination pave the way for transcriptional fine-tuning.
Braatsch S, Helmark S, Kranz H, Koebmann B, Jensen PR.
Biotechniques. 2008 Sep;45(3):335-7.
-The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters.
Jensen PR, Hammer K.
Appl Environ Microbiol. 1998 Jan;64(1):82-7.
-Construction and model-based analysis of a promoter library for E. coli: an indispensable tool for metabolic engineering.
De Mey M, Maertens J, Lequeux GJ, Soetaert WK, Vandamme EJ.
BMC Biotechnol. 2007 Jun 18;7:34.

The first two papers include sequence info of the promoter sets.

Best,

Koen

Dear all,

All the best, Holger

--
Dipl.Biol. Holger Merker

Tel.: +49-(0)3641-571212

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