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Looking for a reference for one of your suggested protocols
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Stefan Moreira
Oct 23, 2008, 12:19:47 PM10/23/08
to biof...@magpie.bio.indiana.edu
Hello Mr. Wright,
I'll begin by apologizing if I have addressed you incorrectly, I came across your name in a rather dated (1997) correspondence with George O'toole when searching for a good way of enumerating the viable cells in a biofilm. The method you describe to Mr. O'toole (employing sonication of cells followeed by dilution) sounds perfect my intended application;enumerating the E. coli cells from a biofilm in a microplate, and was hoping that you may have on hand a reference for the protocol you described. Here is a link to the description you provided http://www.bio.net/bionet/mm/biofilms/1997-April/000106.html. I realize this is a very long shot but figured I would try none-the-less. Thank you very kindly in advance.
Sincerely,
Stefan Moreira H.B.Sc.Bio.
M.Sc. Candidate
Lakehead University
Thunder Bay, ON
807-252-3860
I'll begin by apologizing if I have addressed you incorrectly, I came across your name in a rather dated (1997) correspondence with George O'toole when searching for a good way of enumerating the viable cells in a biofilm. The method you describe to Mr. O'toole (employing sonication of cells followeed by dilution) sounds perfect my intended application;enumerating the E. coli cells from a biofilm in a microplate, and was hoping that you may have on hand a reference for the protocol you described. Here is a link to the description you provided http://www.bio.net/bionet/mm/biofilms/1997-April/000106.html. I realize this is a very long shot but figured I would try none-the-less. Thank you very kindly in advance.
Sincerely,
Stefan Moreira H.B.Sc.Bio.
M.Sc. Candidate
Lakehead University
Thunder Bay, ON
807-252-3860
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