BioTechniques 25(1), July 1998
Mary McCarthy
BioTechniques 25(1), July 1998
Table of Contents
Benchmarks
Preventing Proteolytic Artifacts in the Baculovirus=20
Expression System
L.G. Hom and L.E. Volkman
Nickel-Induced Oligomerization of Proteins Containing=20
10-Histidine Tags
T. Sprules, N. Green, M. Featherstone and K. Gehring
Expression Vector Containing an N-Terminal Epitope Tag for Dictyostelium
discoideum
C.-F. Chen and E.R. Katz
Screening of Recombinant DNA Clones by Single-Colony
Micro-Lysate/Restriction Enzyme
Analysis
K.R. Peterson
Direct Use of Synthetic Peptides for Antiserum Production
N. Sheibani and W.A. Frazier
Subtraction with 3-prime Modified Oligonucleotides Eliminates=20
Amplification Artifacts in DNA Libraries Enriched=20
for Microsatellites
A. Koblizkova, J. Dolezvel and J. Macas
Influence of Magnesium Ion Concentration and PCR=20
Amplification Conditions on Cross-Species PCR
J.J. Ely, A. Reeves-Daniel, M.L. Campbell, S. Kohler and W.H. Stone
Large- and Small-Scale Preparation of Bacteriophage l Lysate
and DNA
M.-T. Su, T.V. Venkatesh and R. Bodmer
Increased Efficiency of Liposome-Mediated Transfection by=20
Volume Reduction and Centrifugation
R.S. Verma, D. Giannola, W. Shlomchik and S.G. Emerson
Microscope Slide for Enhanced Analysis of DNA Damage Using=20
the Comet Assay
M.J. Smith and K.L. O'Neill
The SSCP Phenomenon: Addition of HEPES Buffer Dramatically=20
Affects Electrophoretic Mobility
Q. Liu and S.S. Sommer
Internet On-Ramp
Cyberspace for Biologists
Short Technical Reports
DNA Compression Caused by an Upstream Point Mutation
B.G. Weinshenker, D.D. Hebrink, A.M. Gacy and C.T. McMurray
High-Temperature, Nonradioactive Primer Extension Assay for Determination of=
a
Transcription-Initiation Site
M. Yamada, H. Izu, T. Nitta, K. Kurihara and T. Sakurai
Genotyping of Human Apolipoprotein E Alleles by the New Qualitative,
Microplate-Based
CASSI-Detection Assay
T. Kohler, A.-K. Rost, K. Purschwitz, S. Vondran, H. Remke,=20
O. Wagner and V. Richter
Development of a Yeast Trihybrid Screen Using Stable Yeast=20
Strains and Regulated Protein Expression
K.J. Fuller, M.A. Morse, J.H.M. White, S.J. Dowell and M.J. Sims
Making Genes Green: Creating Green Fluorescent Protein=20
(GFP) Fusions with Blunt-End PCR Products
W. Lo, W. Rodgers and T. Hughes
One-Step Fluorescent Probe Product-Enhanced Reverse=20
Transcriptase Assay
B.A. Arnold, R.W. Hepler and P.M. Keller
Adapting the Biomek=AE 2000 Laboratory Automation=20
Workstation for Printing DNA Microarrays
J. Macas, M. Nouzova and D.W. Galbraith
BioComputing
GeneUp: A Program to Select Short PCR Primer Pairs that=20
Occur in Multiple Members of Sequence Lists
G. Pesole, S. Liuni, G. Grillo, P. Belichard, T. Trenkle,
J. Welsh and M. McClelland
Intensity Modulation of Pseudocolor Images
L.E. Hinman and P.J. Sammak
Research Reports
Direct Quantitation of RNA Transcripts by Competitive Single-Tube=20
RT-PCR and Capillary Electrophoresis
N.D. Borson, M.A. Strausbauch, P.J. Wettstein, R.P. Oda, S.L. Johnston=
=20
and J.P. Landers
Elimination of False Positives Generated Through PCR=20
Re-amplification of Differential Display cDNA
G. Miele, L. MacRae, D. McBride, J. Manson and M. Clinton
Carbohydrate Affinity PAGE for the Study of Carbohydrate-
Binding Proteins
P.D. Rye and N.V. Bovin
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