BioTechniques 24(5), May 1998
Mary McCarthy
BioTechniques 24(5), May 1998
Table of Contents
Benchmarks
Construct for Assessment of Transfected Secretory Proteins Using an
Independently Secreted
Reporter Gene
M. Ailenberg and M. Silverman
On-Line Microdialysis for Mass Spectrometry
S. Konig, S. Weiler and H.M. Fales
Single-Step Purification of T7 RNA Polymerase with a 6-Histidine Tag
T. Ellinger and R. Ehricht
Differential mRNA Display at the Single-Cell Level
C. Renner, L. Trumper, J.-P. Pfitzenmeier, U. Loftin, K. Gerlach, I.
Stehle,
A. Wadle and M. Pfreundschuh
Simplified Method for the Telomeric Repeat Amplification Protocol (TRAP)
L.N. Sellner, P.M. Lock and G.R. Turbett
Artifact Occurring during Combined In Situ Hybridization and
Immunocytochemistry Analysis
because of Positive Chemography
T. Hakkinen and S. Yla-Herttuala
DNase I Treatment of Total RNA Improves the Accuracy of
Ribonuclease Protection Assay
D.A. Dixon, D.L. Vaitkus and S.M. Prescott
Fusion PCR, a One-Step Variant of the Megaprimer Method
of Mutagenesis
C. Karreman
Real-Time Fluorescence Detection of a Single DNA Molecule
C. Lockey, E. Otto and Z. Long
One-Step Procedure for Screening Recombinant Plasmids by Size
E. Beuken, C. Vink and C.A. Bruggeman
Enhanced Efficiency of Cloning FACS-Sorted Mammalian Cells
C. Rodriguez and H.F. Lodish
Isolation of Stable Bacterial Artificial Chromosome DNA Using a
Modified Alkaline Lysis Method
D. Sinnett, C. Richer and A. Baccichet
Rapid Zymogram for Lipase
R.P. Yadav, R.K. Saxena, R. Gupta and W.S. Davidson
Modified Hirt Procedure for Rapid Purification of Extrachromosomal DNA from
Mammalian
Cells
U. Arad
Concentrating Dilute Protein Solutions for Gel Electrophoresis
S. Yitzhaki and J. Sperling
Minimizing False Positives in Differential Display
M.J. Luce and P.D. Burrows
Internet On-Ramp
Cyberspace for Biologists
Short Technical Reports
Vectors for Expressing T7 Epitope- and His6 Affinity-Tagged Fusion Proteins
in S. cerevisiae
S. Enomoto, G. Chen and J. Berman
UGA Read-Through Artifacts - When Popular Gene Expression
Systems Need a pATCH
G. MacBeath and P. Kast
Microsatellite Enrichment in Organisms with Large Genomes
(Allium cepa L.)
D. Fischer and K. Bachmann
Multiwell 14CO2-Capture Assay for Evaluation of Substrate
Oxidation Rates of Cells in Culture
C.L. Collins, B.P. Bode, W.W. Souba and S.F. Abcouwer
Diagnostic Techniques
Quantitative, Competitive PCR Assay for HIV-1 Using a
Microplate-Based Detection System
P.C. Guenthner and C.E. Hart
Fast-Painting of Human Metaphase Spreads Using a Chromosome-Specific,
Repeat-Depleted
DNA Library Probe
M. Durm, L. Schussler, H. Munch, J. Craig, H. Ludwig, M. Hausmann
and C. Cremer
Quick-FISH: A Rapid Fluorescence In Situ Hybridization Technique
for Molecular Cytogenetic Analysis
S.K. Banerjee, A.P. Weston, D.L. Persons and D.R. Campbell
High-Throughput Screening for Known Mutations by Automated
Analysis of Single Sequencing Reactions
T. Kristensen, V.N. Kristensen and A.-L. Borresen-Dale
Detection of Single-Nucleotide Polymorphism
W. Gu, G.D. Aguirre and K. Ray
Doublex Sequencing in Molecular Diagnosis of Hereditary
Diseases
J. Plaschke, H. Voss, M. Hahn, W. Ansorge and H.K. Schackert
Research Reports
3-prime-End cDNA Pool Suitable for Differential Display from a Small
Number of Cells
S. Zhao, G. Molnar, J. Zhang, L. Zheng, L. Averboukh and A.B. Pardee
Development of Competitive mRNA PCR for the Quantification of
Interleukin-6-Responsive junB
Oncogene Expression
P. Igaz, G. Fejer, C. Szalai, S. Toth and A. Falus
E. coli-Based In Vitro Transcription/Translation: In Vivo-Specific
Synthesis Rates and High
Yields in a Batch System
R. Patnaik and J.R. Swartz
Functional Assays to Identify and Characterize Regulators of
Microtubule Behavior
J. McMeekan and P. Wadsworth
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