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BioTechniques 24(6), June 1998

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Mary McCarthy

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May 28, 1998, 3:00:00 AM5/28/98
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BioTechniques 24(6), June 1998
Table of Contents

Benchmarks

Mounting Technique Allows Observation of Immuno-Labeled Cells=20
on Plastic Coverslips
M.F. O'Donohue, T. Cheutin, C. Klein, H. Kaplan and D. Ploton

Double Labeling for Whole-Mount In Situ Hybridization in Mouse
J. Hecksher-S=F8rensen, R.E. Hill and L. Lettice

X-gal Staining of Drosophila Embryos Compatible with Antibody=20
Staining or In Situ Hybridization
M.-T. Su, K. Golden and R. Bodmer

pMECA: A Cloning Plasmid with 44 Unique Restriction Sites that=20
Allows Selection of Recombinants Based on Colony Size
J.M. Thomson and W.A. Parrott

Protection of Proteolysis-Prone Recombinant Proteins in Baculovirus
Expression Systems
H.-W. Grosch and A. Hasilik

Successful Expression of a Selenomethionyl Protein under Control=20
of the Temperature-Sensitive l Repressor Requires Higher than=20
Normal Temperature
A. Witkowski and S. Smith

Genomic DNA Extraction from Small Amounts of Sera to be Used for Genotype
Analysis
Z. Lin and J. Floros

Disposable Device for the Isolation of DNA from Agarose Gels
J.M. Thomson and M.M. Compton


Internet On-Ramp
Cyberspace for Biologists


Short Technical Reports

Quantification of Low-Copy Transcripts by Continuous SYBR Green I
Monitoring during
Amplification
T.B. Morrison, J.J. Weis and C.T. Wittwer

Improving Sensitivity of the Electrophoretic Mobility Shift Assay by
Restricting Tissue
Phosphatase Activities
M.-A. Laniel and S.L. Guerin

Selection Strategy for Site-Directed Mutagenesis Based on Altered=20
b-Lactamase Specificity
C.A. Andrews and S.A. Lesley

Molecular Analysis of Animal and Plant Cells is Facilitated by Their
Attachment to Collodion
(Cellulose Nitrate) Films
E.J. Oakeley, Y. Liu and J.-P. Jost


BioFeedback


BioComputing

Identification and Analysis of Weak Linear Banding Patterns of Fish
Chromosomes with a
Computer-Based Densitometric Method
Q. Zhang and T.R. Tiersch

Emulation of an ELISA Reader and Quantitative Image Analysis Using the
Computer Program
Digital-OD
S. Jansen, B. Kriegesmann and B. Brenig

Correcting Data Shifts in Gel Files Created by Model 377 DNA Sequencers
K.M. O'Brien, M.A. Ironside, M.C. Athanasiou, M.A. Basit, G.A. Evans=20
and H.R. Garner

Reconstructing Fragmented Gel Files Created by Model 377 DNA Sequencers
K.M. O'Brien, J.J. Schageman, G.A. Evans and H.R. Garner

Stereo-4-D Reconstruction and Animation from Living Fluorescent Specimens
W.A. Mohler and J.G. White

Improving Read Lengths by Recomputing the Matrices of=20
Model 377 DNA Sequencers
K.M. O'Brien, J.J. Schageman, T.H. Major, G.A. Evans and H.R. Garner


Cancer Research Techniques

Identification and Verification of Differential Display cDNAs Using=20
Gene-Specific Primers and Hybridization Arrays
K.J. Martin, C.-P. Kwan, M.J. O'Hare, A.B. Pardee and R. Sager

Mutational Analysis Using Enriched PCR and Cycle Sequencing
K.R. Mitchell and D. Warshawsky

Green Fluorescent Protein Tag for Studies of Drug-Induced Translocation of
Nucleolar Protein
RH-II/Gu
B.C. Valdez, L. Perlaky, Z.-J. Cai, D. Henning and H. Busch

Identification of Anti-Invasive but Noncytotoxic Chemotherapeutic Agents
Using the Tetrazolium
Dye MTT to Quantitate Viable Cells in Matrigel
C.Y. Sasaki and A. Passaniti

Product Application Focus

Sequence Analysis of Mitochondrial DNA Hypervariable Regions Using Infrared
Fluorescence
Detection
D.L. Steffens and R. Roy

1998 Authors Index (Volume 24)
New Products
Index to Advertisers


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