BioTechniques 25(3), September 1998
Mary McCarthy
Table of Contents
Benchmarks
Anchored Oligo(dT) Primers for Automated Dye Terminator
DNA Sequencing
W.S. Grayburn and T.L. Sims
Rapid DNA Purification from Agarose Gels Using 96-Well Format,
Centrifuge-Driven Filtration
H. Engkvist and U. Gyllensten
Sodium Vanadate Treatment as a Shortcut Following Alkaline Phosphatase
Cleavage
Y.-Y.P. Wo, D.-S. Sheu and C.-H. Lu
Photographic Recording of Fluorescent DNA Bands on
Agarose Gels
N.C. Mills and X. Zhang
Allele-Specific, Inverse-PCR Amplification for Genotyping
MN Blood Group
Z.-X. Li, S. Yoshimura, T. Kobayashi and A. Akane
PCR-Mediated Mutagenesis in Sequences Recalcitrant to Homogeneous
Amplification
R.V.A. Intine and R.N. Nazar
Repetitive Elements Amplified during Differential Display
I. Steinebrunner, B. Schlierf and G.M. Hocke
Use of Staphylococcus aureus Protein-A Subdomains as a Tag
for the Sensitive Detection of Recombinant Fusion Proteins
M.M. Gosink and R.D. Vierstra
Purification of Ig-Fusion Proteins from Medium Containing Ig
T. Sakurai, C. Roonprapunt and M. Grumet
Inexpensive Motion Detectors for Quantification of Animal
Activity
K.M. Kramer
Simple Blocking System for Use with Maleimide-Labeled Nucleic
Acid Probes
J. Fung, M.M. Moronne and H.-U.G. Weier
Internet On-Ramp
Cyberspace for Biologists
Short Technical Reports
Peak Height Pattern in Dichloro-Rhodamine and Energy Transfer
Dye Terminator Sequencing
H. Zakeri, G. Amparo, S.-M. Chen, S. Spurgeon and P.-Y. Kwok
Rapid Acquisition of Unknown DNA Sequence Adjacent to a
Known Segment by Multiplex Restriction Site PCR
K.L. Weber, M.E. Bolander and G. Sarkar
Highly Sensitive Northern Hybridization Using a Rapid Protocol
for Downward Alkaline Blotting of RNA
I.L. Ingelbrecht, C.I. Mandelbaum and T.E. Mirkov
Limitations for Purification of Murine Interleukin-18 When
Expressed as a Fusion Protein Containing the FLAG Peptide
A. Elhofy and K.L. Bost
Isolation of Differentially Expressed Genes by Combining Representational
Difference Analysis
(RDA) and cDNA Library Arrays
M. Geng, C. Wallrapp, F. Muller-Pillasch, M. Frohme, J.D. Hoheisel and
T.M. Gress
Fission Yeast Expression Vectors Adapted for Positive
Identification of Gene Insertion and Green Fluorescent
Protein Fusion
Y. Zhao, R.T. Elder, M. Chen and J. Cao
Research Reports
Quantitation of Mixed-Base Populations of HIV-1 Variants
by Automated DNA Sequencing with BODIPY Dye-
Labeled Primers
M.L. Metzker, M.A. Ansari-Lari, X.-M. Liu, D.J. Holder and R.A. Gibbs
Large DNA Fragment Sizing Using Native Acrylamide Gels on
an Automated DNA Sequencer and GENESCAN Software
C.R.E. McEvoy, R. Seshadri and F.A. Firgaira
DAF Optimization Using Taguchi Methods and the Effect of
Thermal Cycling Parameters on DNA Amplification
G. Caetano-Anolles
Selective Propagation of Retinal Pericytes in Mixed Microvascular
Cell Cultures Using L-Leucine-Methyl Ester
C.S. Lee, W.F. Patton, N. Chung-Welch, E.T. Chiang, K.H. Spofford
and D. Shepro
DNA Nicks and Increased Sensitivity of DNA to Fluorescence
In Situ End Labeling during Functional Spermiogenesis
A. Smith and T. Haaf
RNA Internal Standards Quantify Sensitivity and Amplification Efficiency of
Mammalian Gene
Expression Profiling
R.D. Madison and G.A. Robinson
Product Application Focus
Mutation Typing Using Electrophoresis and Gel-Immobilized Acrydite Probes
M. Kenney, S. Ray and T.C. Boles
Measurement of Isolated Myocyte Volume Using the Coulter
Models Z2 and ZM/C256: A Comparison of Instrument
Function
S. Said, T. Tamura and A.M. Gerdes
New Products
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