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Macrophage activation marker?

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tr...@my-dejanews.com

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Jun 3, 1998, 3:00:00 AM6/3/98
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Hi!

I would need to isolate LPS-activated mouse macrophage cells
(RAW or J774) by magnetic separation with antibodies. For this,
I would need a cell surface antigen that has low or undetectable basal
expression level in resting macrophage but upregulated expression
in activated macrophage. I have tried already CD14, Mac-1 and
CD25. Trouble with CD14 and Mac-1 is quite that they have high basal
expression levels based on FACS analysis. I did not see any CD25
expression at all.

Any information is appreciated.

Thanks!

Tapani Ronni, PhD
Howard Hughes Medical Institute/UCLA School of Medicine

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mark

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Jun 4, 1998, 3:00:00 AM6/4/98
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I would look into cd 80 and/or 86 and do some proof of concept stuff by
staining after activating some isolated MQs
markH

Hao Xiao

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Jun 5, 1998, 3:00:00 AM6/5/98
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I experience non-specific binding in western-blotting system of mouse tissue
samples

My primary Abs are immunoaffinity purified rabbit Ab against pY, ERK1, P38
(hog1) and p53. All my blots contained nastic bands corresponding to
immunoglobulins. I thought it is attributed to the cross-reactivities of
anti-rabbit Ig's to mouse Igs. But the bands are intensified through
increasing the conc. of primary Ab but not the secondary. Pleasae help.

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