Non radioactive Cytotoxic activity assay

[Eniafe, Rhoda (NIH/NHLBI) [E]]

Hello David,

I have been try to use Calcein-AM in Cytotoxic assay, but I am having
problem with leakage and inconsistencies with the killing assay, do you
encounter this kind of problem(s) and what are the steps you take to
prevent it.

Thanks

Rhoda

Rhoda Eniafe, MT(ASCP)
Stem Cell Allogeneic Transplantation Section
HEMATOLOGY BRANCH,NHLBI,NIH
Mail address: Bldg 10 (CRC), Rm. 3E-5288, MSC 1202
Office address: Bldg 10 (CRC), Rm. 3E-5288
Phone# 301-435-2624; Fax# 301-480-2664
email: eni...@nhlbi.nih.gov