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Direct ELISA

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Raghunath

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Nov 29, 2006, 5:20:23 AM11/29/06

to Imm...@magpie.bio.indiana.edu

Hello everybody,

I am trying to standardise Direct ELISA by coating
protein as a positive control using
carbonate-bicarbonate buffer(pH 9.6),blocking with
3%BSA and then reacting with monoclonal antibody.It is
working well with standards but when i apply sample
signal/noise ratio is high and
some times negative samples are giving higher OD than
positive samples.Can u pls suggest me what could be
the reason

Thank you,
Raghunath

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