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Glycoprotein turn-over analysis
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Vladimir Gainullin
Aug 17, 2012, 2:19:43 AM8/17/12
to glyc...@magpie.bio.indiana.edu
I was recently able to estimate a post-Golgi turn-over rate of a
glycoprotein by perturbing the Golgi mannosidase II with Swainsonine, then
following the remaining Endo-H resistant form of my favorite glycoprotein
using western blotting. Although I did not see this being used elsewhere
in the literature I assume that someone must have done this previously.
Does this method have significant drawbacks compared to
cycloheximide-chase?
Thank you
glycoprotein by perturbing the Golgi mannosidase II with Swainsonine, then
following the remaining Endo-H resistant form of my favorite glycoprotein
using western blotting. Although I did not see this being used elsewhere
in the literature I assume that someone must have done this previously.
Does this method have significant drawbacks compared to
cycloheximide-chase?
Thank you
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