Regarding your question on Agrobacterium transformation
shahzad anjam
I am writing regarding your question about Agrobacterium mediated transformation. As I have experienced after 48 hours of co-cultivation, the gene expression starts. As soon as the gene enters in the nucleus its transcription starts. Moreover its further expression depends upon the integration position of gene in the chromosome. I have observed that in gene gun transformation, next day expression was observed.
This is my understanding on the topic.
Regards
Muhammad Shahzad Anjam
Lecturer Biotechnology, Institute of Biotechonology ,
Bahauddin Zakariya University , Multan (www.bzu.edu.pk)
Pakistan .
Contact Details:
Office (Phone) +92(0)61 9210071-4, Ext: 1924, 3900
Cell Phone: +92-333-8174001
E-mail: shahza...@bzu.edu.pk
E-mail: shahz...@yahoo.com
Get your preferred Email name!
Now you can @ymail.com and @rocketmail.com.
http://mail.promotions.yahoo.com/newdomains/aa/