alternatives to Halocarbon oil from sigma for microinjections

[Rohit Joshi]

Hi All
I am looking for an alternative source of Halocarbon oil for Drosophila
microinjections from Sigma.

Since we have been having problems getting it from Sigma, therefore we are
forced to look for alternatives sources of the same.

I will appreciate any information on any other commercial source (equally
competitive in price as Sigma or cheaper) for halocarbon oil or any other
alternative oil which can be used for Drosophila embryonic microinjections
for making transgenics.

Thanks
Regards and best wishes for 2014

Rohit Joshi


--
रोहित जोशी, सीडीएफडी-हैदराबाद, भारत.
--
Dr. Rohit Joshi, Head-LDND
CDFD, 4-1-714, Tuljaguda Complex,
Mozamzahi Road, Nampally, Hyderabad
Andhra Pradesh-500001
INDIA.

Phone: +91-40-24749354

[Schulze, Karen Lynn]

I have been ordering direct from the manufacturer. We use quite a lot so I order the 2 kg size at $720 USD (not including shipping).

I don't know a lot about oil and why this one in particular works or how it was selected for this purpose. It is just the one that people always used. Maybe baby oil or light mineral oil would also work, although I have never tested them.

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[Graham Thomas]

Halocarbon (perfluorocarbon) oils have a very high dissolved oxygen
content - THIS is why they are used for immersing fly embryos (and for
liquid ventilation in the medical field). I don't think you want to go
using these other alternatives.

cheers!
Graham

--
Center for Cellular Dynamics
Departments of Biology and of Biochemistry and Molecular Biology
The Pennsylvania State University, University Park
510 Mueller Lab,
University Park, PA 16802 USA

bmb.psu.edu/directory/gxt5

Please pass on these links to aspiring graduate students:
huck.psu.edu/education/cell-and-developmental-biology
huck.psu.edu/education/genetics
bio.psu.edu/graduate-portal/join-our-program
bmb.psu.edu/graduate/how-to-apply

[Mark Thorson]

Graham Thomas wrote:
>
> Halocarbon (perfluorocarbon) oils have a very high dissolved oxygen
> content - THIS is why they are used for immersing fly embryos (and for
> liquid ventilation in the medical field). I don't think you want to go
> using these other alternatives.

Fluorinert is a 3M brand name for perfluorinated oil.
It's often available on eBay.

[ITO, Kei]

Dear fellow insect brain researchers

Many of you may know that the Insect Brain Name Working Group
has been discussing a systematic way to document various regions
and structures of the insect brain. The final version of the proposal
was published in the Feb. 19 issue of Neuron. (See the following link)

A Systematic Nomenclature for the Insect Brain
http://www.sciencedirect.com/science/article/pii/S0896627313011781
(The paper will become open access after six months, i.e., in August.)

The main part of the proposal (84 pages) is provided as Supplemental
Information (Document S1) in the above site, together with serial
section movies and interactive 3D clickable maps.

[Neuropil map]
To enable unambiguous documentation of locations in the brain, we
defined unit neuropils and their boundaries throughout the brain. We
employed the brain of Drosophila melanogaster as a framework, but
we tried to make it a useful reference landmark for analyzing brains
of many other insects.

The neuropil map is already used as a basis for documenting projection
patterns of neurons labeled by expression driver strains (e.g., Janelia
Farm FlyLight project, http://flweb.janelia.org/cgi-bin/flew.cgi) and
for comparing projections of identified neurons and neuron groups
(e.g., The Virtual Fly Brain, http://www.virtualflybrain.org/).

[Template of the neuropil map]
For those who want to map the neurons they identified onto the defined
neuropil map using 3D registration, raw voxel data of the template brain
and neuropil boundaries are available via the FlyBase ftp site.
ftp://ftp.flybase.org/flybase/associated_files/InsectBrainNomenclature_RawData.zip
(Caution: 300 megabytes. Do not download unless you are familiar with
software such as Amira, FIJI, CMTK, or BrainAligner.)

[Conflicting terms]
We also provide a list of conflicting terms and their solutions (pp. 10-15
of the above document) to resolve problems such as inconsistent terms
used in different species or by researchers, as well as the terms that
may cause unexpected misunderstanding by vertebrate neuroscientists.


[Thank you all.]
Throughout the activity of the working group since 2007, intermediate
versions of the nomenclature were presented at many meetings and
symposia. We received plenty of helpful suggestions and comments
and tried to incorporate them as much as possible. Without such broad
inputs, the current nomenclature would not have been completed. We
appreciate the support and suggestions by the community and hope
that the framework should serve as helpful communication tools for a
broad field of insect and arthropod neurosciences.


Insect Brain Name Working Group
Kei Ito, Kazunori Shinomiya, Masayoshi Ito, J. Douglas Armstrong,
George Boyan, Volker Hartenstein, Steffen Harzsch, Martin Heisenberg,
Uwe Homberg, Arnim Jenett, Haig Keshishian, Linda L. Restifo,
Wolfgang Rossler, Julie H. Simpson, Nicholas J. Strausfeld, Roland
Strauss, and Leslie B. Vosshall