Chlamy toxin

[KAUR, SIMRAT]

Hi Chlamy researchers!



I visited the Chlamy connection website to connect to the Chlamy research community.


I am working on Haematococcus pluvialis (HP) cultivation in an open PBR system where I frequently encounter the problem of chlamy contamination. I have read that Chlamy release fatty acid like toxin which inhibit the growth of HP. I tried formalin to kill motile chlamy cells and have been successful but anticipate that this treatment is affecting HP as well.


Can you suggest ways in which I can control chlamy contamination in HP cultures.

Another question is how does chlamy get into HP culture? Is it air borne or water borne or was in the stock culture?

I received the HP stock culture from SSCAP which was free of any chlamy cells (or i was unable to detect).


Any suggestions would be highly appreciated.

?Many Thanks!



Regards
Simrat Kaur
PhD Scholar
Botany & Plant Science
NUIG

[Ben Engel]

Hi Simrat,

I have never heard of flying chlamy, so either your starting culture was contaminated (that would be my guess) or you have contaminated media or flasks.

I don't know anything about growing HP, but can it be grown on solid media (in a Petri dish)? If so, I would recommend streaking very dilute culture onto a plate so that single clonal colonies grow up. Then you could pick HP colonies away from any contaminating chlamy.

If you still get contamination after that, I would suspect your media or flasks. And I would give everything a good autoclaving.

Good luck!
Ben

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[David L Herrin]

Simrat,
Chlamydomonas sp. have been detected in airborne sampling along with a
bunch of other green algae, cyanobacteria and diatoms. You may have to do
some basic analysis of the one your getting, e.g. whether it can grow
on nitrate as sole nitrogen source, etc. in order to control it. Can you
determine the species?
David Herrin