IPs

[rm]

There are two possible solutions I am aware of:
You can try to couple the Ab which you use for the IP covalently to the
beads. Protocol is in the Antibodybook by Harlow and Lane.
Or you can use protein A instead of your secondary Ab. It does not bind to
the denatured Ab on the blot.
It binds well to rb, mo IgG2a and IgG2b. For IgG1 you might want to try
Protein G (no experience).

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Rudolf Meili
Department of Biology
Center for Molecular Genetics, Rm.228
University of California, San Diego
9500 Gilman Drive
La Jolla, CA 92093-0634
U.S.A.
fax: +1 (619) 534-7073
phone: +1 (619) 534-4825.
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