I have the same question as Matthew. We have recently mutated the Lys of
subdomain II to Asn. To our surprise, our kinase is still alive. This
experiment is done in E. coli in the presence of radioactive phosphate. If
anyone can come up with an explanation, that will help us a lot.
Best regards
Cheng-Cai Zhang
Cheng-Cai Zhang (Ph.D)
Ecole Superieure de Biotechnologie de Strasbourg
Universite Louis Pasteur de Strasbourg
Boulevard Sebastien Brandt
F-67400 Illkirch, France
Phone: +33 3 88655290
Fax: +33 3 88655330