Anatol Kolomeisky: Molecular Mechanisms of Precise Timing in Cell Lysis. 10th Dec, 2024 from 4:00-5:00 pm IISER pune Seminar room 33

[Dr. Chaitanya Athale]

Hi all

Talk of some relevance to this group.

Those in Pune pls take note. 

Speaker: Prof. Anatoly Kolomeisky

Affliation: Rice University, USA 

Title: Molecular Mechanisms of Precise Timing in Cell Lysis

Date and time 📅 10th Dec, 2024 from 4:00 

• 5:00 pm

Venue: 📌 Seminar Room 33

☕Tea/Coffee will be served at 3:45 pm

Abstract

Many biological systems exhibit precise timing of events, and one of the most known examples is cell lysis, which is a process of breaking bacterial host cells in the virus infection cycle. However, the underlying microscopic picture of precise timing remains not well understood. We present a novel theoretical approach to explain the molecular mechanisms of effectively deterministic dynamics in biological systems. Our hypothesis is based on the idea of stochastic coupling between relevant underlying biophysical and biochemical processes that lead to noise cancellation. To test this hypothesis, we introduced a minimal discrete-state stochastic model to investigate how holin proteins produced by bacteriophages break the inner membranes of gram-negative bacteria. By explicitly solving this model, the dynamic properties of cell lysis are fully evaluated, and theoretical predictions quantitatively agree with available experimental data for both wild-type and holin mutants. It is found that the observed threshold-like behavior is a result of the balance between holin proteins entering the membrane and leaving the membrane during the lysis. Theoretical analysis suggests that cell lysis achieves precise timing for wild-type species by maximizing the number of holin proteins in the membrane and narrowing their spatial distribution. In contrast, for mutated species, these conditions are not satisfied. In addition, we investigated what physicochemical properties of holin proteins are the most relevant for cell lysis by employing statistical correlation analysis for different experimentally observed mutant species. Our findings reveal significant correlations between various physicochemical features and cell lysis dynamics. Notably, we uncover a strong inverse correlation between local hydrophobicity and cell lysis times, underscoring the crucial role of hydrophobic interactions in membrane disruption. Stimulated by these observations, a predictive model capable of explicitly estimating cell lysis times for any holin protein mutants based on their mean hydrophobicity values is developed. Our theoretical approach presents a possible general molecular picture of precise dynamic regulation in intrinsically random biological processes.

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Prof. Chaitanya A. Athale PhD,

Div of Biology, IISER Pune,

India

http://www.iiserpune.ac.in/~cathale