Greetings of the day!!!
I have a small query on NCI60 cell line expression with specific target gene. I downloaded the NCI60 data from your downloads option. I have found there is variation in expression of 1007_s_at (probe) from the file I have downloaded and results displayed online Biogps, not only this specific gene mentioned but all the probes there is a variation from the file downloaded and displayed online. It would be great if you can help on this
Thanks in advance
Notice: The information contained in this electronic mail message is intended only for the use of the designated recipient. This message is privileged and confidential and the property of GVK BIO or its affiliates and subsidiaries. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, then you are hereby notified that you have received this message in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. In such an instance, please notify us immediately by telephone +91-40-66929999 and destroy any and all copies of this message in your possession (whether hard copies or electronically stored copies).
Thanks for your kind help. Much appreciated
I have two more questions regarding NCI60 cell lines data
Ø Is normalization required for this NCI60 or Is it ok if I consider only raw values given because Human U133A/GNF1H Gene Atlas is normalized with gcmra, so is it necessary to do normalization for NCI60 cell lines expression data
Ø Can you please send the information on cell lines as NCI60_sample_info.xls (92 KB) only contains information about 56 cell lines rest of them are missing. Ex: huh-7,hek293 cell lines doesn’t contain any information like cell type, tissue type, etc,. in sample_info excel sheet.
Thanks in advance!!
From: Rajasekhar Moturi
Sent: Monday, August 08, 2016 9:40 AM
To: 'G Tsueng'
Subject: RE: NCI60
Thanks a ton for your kind reply with great explanation. Understood
Is normalization required for this NCI60 or Is it ok if I consider raw values because Human U133A/GNF1H Gene Atlas is normalized with gcmra.
Thanks in advance
From: G Tsueng [mailto:gts...@gmail.com]
Sent: Thursday, August 04, 2016 11:37 PM
Subject: Re: NCI60