Question regarding the HEK 293 cell lines in the NCI60 expression data
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Fred
Nov 26, 2009, 8:09:18 AM11/26/09
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Hello BioGPS Team,
I was wondering what is the difference between these three lines
available in the expression chart for the NCI60 dataset: HEK2 93; HEK
293 T, HEK 293 T-rex
Thanks in advance,
Fred
I was wondering what is the difference between these three lines
available in the expression chart for the NCI60 dataset: HEK2 93; HEK
293 T, HEK 293 T-rex
Thanks in advance,
Fred
Matt
Nov 29, 2009, 2:42:33 PM11/29/09
to BioGPS
T is for "T antigen" expression (likely used in original
transformation?), not sure about the rex.
transformation?), not sure about the rex.
Andrew Su
Dec 1, 2009, 2:29:51 PM12/1/09
to Fred, BioGPS
Hi Fred,
HEK 293T are HEK 293 cells transformed by expression of the large T antigen from SV40 virus that inactivates pRb. HEK 293 Trex stably express the tetracycline repressor protein.
Hope that helps...
-andrew
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HEK 293T are HEK 293 cells transformed by expression of the large T antigen from SV40 virus that inactivates pRb. HEK 293 Trex stably express the tetracycline repressor protein.
Hope that helps...
-andrew
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Eugene
Dec 7, 2009, 11:25:16 AM12/7/09
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HEK293 were originally established by transfecting human embryonic
kidney cells with sheared adenoviral DNA. The cells express several
adenoviral proteins, including the oncogenes responsible to
inactivation of p53 and pRB. They do not make adenenovirus, but
support replication of adenoviral vectors.
HEK293T have been additionally transfected with an expression
construct for large-T antigene of SV40 virus. Several cell lines were
established this way. The chances are, you have the one that was
established with the temperature-sensitive large T. Large T affects a
multitude of processes in a cell, and some of its functions duplicate
those of adenoviral oncogenes. However, it is also a transcription
factor and a replication factor for SV40 in primate cells. The
original intent was to have a system where transcription and/or
replication could be regulated in mammalian cells in a LgT-dependent
manner. Superb transfection efficiency is what made these cells
popular.
T-REx™-293 are a commercial product of Invitrogen. They were derived
from HEK293 by stable expression of and expression cassette for the
modified tet-repressor protein (from pcDNA6/TR plasmid).
Eugene.
kidney cells with sheared adenoviral DNA. The cells express several
adenoviral proteins, including the oncogenes responsible to
inactivation of p53 and pRB. They do not make adenenovirus, but
support replication of adenoviral vectors.
HEK293T have been additionally transfected with an expression
construct for large-T antigene of SV40 virus. Several cell lines were
established this way. The chances are, you have the one that was
established with the temperature-sensitive large T. Large T affects a
multitude of processes in a cell, and some of its functions duplicate
those of adenoviral oncogenes. However, it is also a transcription
factor and a replication factor for SV40 in primate cells. The
original intent was to have a system where transcription and/or
replication could be regulated in mammalian cells in a LgT-dependent
manner. Superb transfection efficiency is what made these cells
popular.
T-REx™-293 are a commercial product of Invitrogen. They were derived
from HEK293 by stable expression of and expression cassette for the
modified tet-repressor protein (from pcDNA6/TR plasmid).
Eugene.
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