Hi all,
I've started generating a few plots following the notes of the meeting, and storing them in a separate Google doc, to avoid overloading the main one.
https://docs.google.com/document/d/1Pe0EKQAGkT2-FpYdNAxbgYkaW-LhU3YFHVIpM3exVJo/edit?usp=sharing I'm also storing the code that I've used at
https://github.com/kevinrue/bioc-teaching-intro for full reproducibility. (I've hit a timeout using GEOquery where I've had to run a few Shell commands manually, but I've included those in a Shell script in the repo too).
In the notes of the meeting, "Lines showing expression of genes over time." makes total sense, but is slightly complicated for single cell, as individual cells were not tracked over time.
At best I can draw a smooth trend. Have a look at the plots and you'll see what I mean.
One more thing: I only plot counts on a log axis, to avoid any transformation of the data at this point, which means it's not properly normalised. I understood that for the purpose of an introduction lesson, that's what people would be working with.
Happy to hear feedback any time.
I'll kick that along as I get the chance.
Best
Kevin