normalized count to Deseq2

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pshtiwan babane

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Dec 24, 2024, 2:55:56 AM12/24/24
to bioconductor-teaching
Hello,
Is there anyone here help me how input a dataset of normalized count data to Deseq2.
Just I have normalized count I don`t know is (FPKM or TPM ) and what packages was used for alignment  .
is possible to find to know is fpkm or tmp?

best

Laurent Gatto

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Dec 24, 2024, 3:52:27 AM12/24/24
to pshtiwan babane, bioconductor-teaching
This mailing list is reserved for teaching related discussions, not
for general questions. Please use the support site for that
https://support.bioconductor.org/.

But I can already confirm that you should never use normalised counts
as input for DESeq2.

Best wishes,

Laurent
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pshtiwan babane

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Dec 24, 2024, 9:20:45 AM12/24/24
to Laurent Gatto, bioconductor-teaching

Thank you for your answer.

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