New in Bio-protocol: October 2025 Updates

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Latest Issue: Oct 20, 2025 Articles published in October Articles in Press Archive

Bio-protocol: October 5 and October 20 issues

Volume: 15, Issue: 20 Oct 20, 2025

Cancer Biology

Combining Microwave Ablation With CAR-T-Cell Therapy in Tumor-Bearing Mouse Models

Authors: Bihui Cao, Gabrielle L. Wheeler, Jensen Mast, Qi Zhao, Jia Shen

Cell Biology

Preparation of Chromatin Fragments From Human Cells for Cryo-EM Analysis

Authors: Suguru Hatazawa, Yoshimasa Takizawa, Hitoshi Kurumizaka

Studying Cargo Transport Using RudLOV

Authors: Tatsuya Tago, Takunori Satoh, Akiko K. Satoh

Generation of Insulin-Producing Alpha TC1-6 Cells Using EpiCRISPR System for Targeted DNA Methylation

Authors: Marija B. Đorđević, Stefan Ž. Marković Hadžić, Jovana J. Rajić, Ana D. Sarić, Tijana R. Marković, Svetlana S. Dinić, Aleksandra S. Uskoković, Nevena M. Grdović, Mirjana V. Mihailović, Jelena D. Arambašić Jovanović, Melita S. Vidaković

Immunology

High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood

Authors: Ilyssa E. Ramos, Teresa S. Hawley, Kevin Rose, Brynja Matthiasardottir, Rohit Farmer, Kyu Lee Han, Michal Toborek, Iyadh Douagi, Georgette N. Jones, James M. Cherry

Identification and Sorting of Adipose Inflammatory and Metabolically Activated Macrophages in Diet-Induced Obesity

Authors: Dan Wu, Komal Rawal, Venkateswararao Eeda, Hui-Ying Lim, Weidong Wang

Molecular Biology

Ribozyme-Mediated Knockdown of lncRNA Gene Expression in Drosophila

Authors: Kevin G. Nyberg, Richard W. Carthew

Efficient circRNA Detection Using the Processive Reverse Transcriptase uMRT

Authors: Ruben Warkentin, Anna Marie Pyle

SunTag-Based Single-Molecule Translation Imaging in Caenorhabditis elegans

Authors: Elise van der Salm, Stefanos Kontogiannis, Suzan Ruijtenberg

Plant Science

Effective Gene Silencing in Plants by Synthetic Trans-Acting siRNAs Derived From Minimal Precursors

Authors: Adriana E. Cisneros, Ana Alarcia, María Juárez-Molina, Alberto Carbonell

Direct Plant Regeneration From Immature Male Inflorescence of Banana (Musa spp.)

Authors: Pradeep Chand Deo

Stem Cell

Rapid and Simplified Induction of Spinal Motor Neurons From Human Induced Pluripotent Stem Cells

Authors: YaoYao Chen, Fan Yang, Timothy O'Brien, Sanbing Shen, Meimei Yang

Volume: 15, Issue: 19 Oct 5, 2025

Bioinformatics and Computational Biology

Image-Based Profiling in Live Cells Using Live Cell Painting

Authors: Thaís Moraes-Lacerda, Mariana Rodrigues-Da-Silva, Shantanu Singh, Marcelo Bispo De Jesus

Biological Engineering

A Protocol Guide to Micro Milling for Bio-Microfluidics

Authors: Hannah L. Viola, Vishwa Vasani, Shuichi Takayama

Artificial Metalloenzymes in Artificial Sanctuaries Through Liquid–Liquid Phase Separation

Authors: Kaixin Wang, Guangjie Zhang, Lei Zhang, Yugang Bai, Tong Wu

Cancer Biology

Generation of Agarose-Based FFPE Cancer Organoids for Morphology Preservation

Authors: Mi Rim Lee, Sumin Kang, A-Ra Jeon, Sung-Woen Choi, Sun-Young Kong, Yun-Hee Kim

Standardized Culture of Skin Fibroblasts From Punch Biopsies for Germline DNA Isolation in Myeloid Malignancies: A Practical Bedside-to-Laboratory Approach

Authors: Parampreet Kour, Nedhi Kumari, Naveen Kaushal, Tanvi Sharma, Shano Naseem, Jogeshwar Binota, Manupdesh Singh Sachdeva, Arihant Jain, Manish Rohilla, Reena Das, Pankaj Malhotra, Pulkit Rastogi

Cell Biology

Rapid and Uniform NHS-Ester-Based Membrane Protein Labeling of Live Mammalian Cells

Authors: Alyssa Burgess, Hirushi Gunasekara, Ying S. Hu

Fluorescence Lifetime-Based Separation of FAST-Labeled Cellular Compartment

Authors: Aidar R. Gilvanov, Ilya D. Solovyev, Alexander P. Savitsky, Mikhail S. Baranov, Yulia A. Bogdanova

Molecular Biology

Assessing Temperature-Dependent DNA Cleavage by CRISPR-Cas9

Authors: Alexa L. Knight, Jinping Luo, George P. Lisi

Plant Science

Detection of Plant RNA–Protein Interactions Using GFP-tag for Immunoprecipitation

Authors: Fernanda Marchetti, Ayelen Distéfano, Gabriela Pagnussat, Eduardo Zabaleta

Advancing 2-DE Techniques: High-Efficiency Protein Extraction From Lupine Roots

Authors: Sebastian Burchardt, Patrycja Wojtaczka, Agata Kućko, Maciej Ostrowski, Emilia Wilmowicz

Quantitative Estimation of Auxin, Siderophore, and Hydrogen Cyanide Production in Halo and Drought-Tolerant Bacterial Isolates for Cucumber Growth

Authors: Zeinab Fotoohiyan, Ali Salehi Sardoei

Stem Cell

Generation of Intestinal Epithelial Monolayers From Single-Cell Dissociated Organoids

Authors: Neta Felsenthal, Danijela Matic Vignjevic

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