Fwd: New in Bio-protocol: March 2026 Updates

[Prash]

 

 

View in browser

Latest Issue: Mar 20, 2026 Articles published in March Articles in Press Archive

Bio-protocol: March 5 and March 20 issues

Volume: 16, Issue: 6 Mar 20, 2026

Bioinformatics and Computational Biology

A Bioinformatics Workflow to Identify eccDNA Using ECCFP From Long-Read Nanopore Sequencing Data

Authors: Wang Li, Biyuan Miao, Shaogui Wan

Cancer Biology

A Simple and Cost-Effective Method for Generating Spheroids From Triple-Negative Breast Cancer Cell Line (MDA-MB-231)

Authors: Ramón Cervantes-Rivera, Luisa Nirvana González-Fernández, Atalia Ziret Romero Rosas, Sandra Jetsamari Figueroa Ortíz, Alejandra Ochoa-Zarzosa, Joel E. López-Meza

Mag-Net Strong Anion Exchange Enables Isolation of Ovarian Cancer Ascites Extracellular Vesicles for Proteomic Biomarker Discovery

Author: Tyler T. Cooper

Cell Biology

Radial Profile-Based Quantification of Centrosomal Proteins

Author: Alan Wainman

Microbiology

A Novel Sequencing Method for Quantification of ZIKV RNA in Individual Cells

Authors: Min Hao, Yisong Wang, Dianyi Du, Wenrong Yang, Qiuzhe Guo, MingJing Tang, Libo Liu, Wei Yang, Yuxuan Liu, Chunyuan Luo, Jing Chen, Peigang Wang, Jing An, Yang Zhou

Molecular Biology

A Cytosine Deaminase–Based Genomic Footprinting Assay (cFOOT-seq) for Detecting Transcription Factor Occupancy

Authors: Meng-Chen Yang, Ang Wu, Heng Wang, Xiaoyu Liu, Jiejun Shi, Shaorong Gao, Jia-Min Zhang

Tandem RNA and Protein Extraction: A Platform for Maximizing the Use of Limited Ex Vivo Tissue Samples

Authors: Ciarán Kennedy, Braden Millar, Luke J. Conroy, Mariam Marai, Mary Barry, Martin O’Donohoe, Orina Belton, Eoin Brennan, Catherine Godson, Monica de Gaetano

Plant Science

A Guide to Reproducible Cellulose Synthase Density and Speed Measurements in Arabidopsis thaliana

Authors: Jan Y. Xue, Shawn D. Mansfield, A. Lacey Samuels, Arun Sampathkumar, René Schneider

A Rapid and Visual Soybean Hairy Root Transformation Protocol Using the RUBY Reporter

Authors: Zhirui Zhang, Qiushi Wang, Yu Geng, Jiuhai Zhao

Fluorescence-Based Absent Allele-Specific Amplification (FAASA) for High-Throughput Detection of Absent Alleles

Authors: Katherine L.D. Running, Sudeshi Seneviratne, Zengcui Zhang, Gurminder Singh, Jason D. Fiedler, Justin D. Faris

Controlled Transmission of a Fijivirus Under Field Conditions Using Mass-Reared Planthoppers

Authors: Analía D. Dumón, Milene R. Barcenilla, Julieta B. Bariles, Nicolás A. Pereyra, Sandra M. Rodriguez, María F. Mattio

Volume: 16, Issue: 5 Mar 5, 2026

Biochemistry

Purification of the Active-State G Protein-Coupled Receptor ADGRL4 for Cryo-Electron Microscopy Using a Modular Tag System and a Tethered mini-G_q

Authors: David M. Favara, Christopher G. Tate

aGPCR-HEK: A Stable High-Expression Inducible Mammalian Cell Expression System for Adhesion GPCR Structural Biology Applications

Authors: David M. Favara, Christopher G. Tate

Bioinformatics and Computational Biology

Machine Learning-Assisted Quantification of Organelle Abundance

Authors: Alexander James Long, Diogo Candeias, Nicki Frederick Coveña, Luc Reymond, Milena Schuhmacher, Stephan Kemp, Noémie Hamilton, Triana Amen

High-Resolution Quantification of Two-Way Nanobody Synergy Using Automated Liquid Handling and Computational Modeling

Authors: Natasha M. Bourgeois, Satchel W. Bell, Jean Paul Olivier, Michael P. Rout, John D. Aitchison, Fred D. Mast

Spatial Proteomics Using S4P

Authors: Ritian Qin, Fuchu He, Weijie Qin

Employing Tribe to Study RNA Interactions of Ataxin-2 in Drosophila S2 Cells

Authors: Shiva Kumar, Omkar Koppaka, Khushboo Agrawal, Baskar Bakthavachalu

Cancer Biology

Non-Enzymatic Isolation of Cancer-Associated Fibroblasts From Human Prostate Tumor Explants

Authors: Giulia Gangarossa, Caterina Grillo, Sara Roccabianca, Erica Pranzini, Marta Iozzo, Giacomo Venditti, Greta Bertoli, Luigi Ippolito, Elisa Giannoni, Giuseppina Comito, Paola Chiarugi

Cell Biology

A Rapid and High-Recovery Extracellular Vesicle (EVs) Isolation Technique from Blood Samples

Authors: Aryan N. Sankpal, Narendra V. Sankpal

Quantifying Lysosomal Degradation of Extracellular Proteins With a Fluorescent Protein-Based Internalization Assay

Authors: Sayana Bun, Kanna Kamikawa, Akira Matsuura, Eisuke Itakura

Obtaining Chondroprogenitors (Articular Cartilage-Derived Cells) via Explant Methodology

Authors: Débora Levy, Pedro Nogueira Giglio, Fabio Alessandro de Freitas, Sergio Paulo Bydlowski, Marco Kawamura Demange

Microbiology

Dynamic Mapping of RNA-Binding Proteins During Bacillus subtilis Sporulation Using Orthogonal Organic Phase Separation

Authors: Thomas Kaboré, Clémentine Delan-Forino

A Standardized Culture Medium for Comparative Drug Efficacy Evaluation Across Plasmodium and Babesia Species

Authors: Pratap Vydyam, Choukri Ben Mamoun

Framework for Analyzing the Anti-biofilm and Anti-virulence Activities of Fatty Acids from Hermetia illucens Larvae Targeting Multidrug-Resistant Klebsiella pneumoniae

Authors: Heakal Mohamed, Elena Marusich, Sergey Leonov

Molecular Biology

Selective Isolation of TOP3B•mRNA Covalent Intermediates Using Denaturing Oligo-dT Pulldown

Authors: Julia E. Warrick, Michael G. Kearse

Neuroscience

Reconstruction of Axonal Projections of Single Neurons Using PointTree

Authors: Lin Cai, Xuzhong Qu, Junwei Wang, Yuan Shen, Tingwei Quan

Combining Suction-Pipette Spectral Identification With Single-Cell RT-PCR to Make Differential Analyses of Amphibian Red and Green Rods

Authors: Olga V. Chernyshkova, Darya A. Nikolaeva, Darya A. Meshalkina, Valeria I. Veligura, Michael L. Firsov

Plant Science

Simple Induction and Detection of Anthocyanins in Arabidopsis thaliana: A Tool for Mutant Screening and Metabolic Analysis

Authors: Yanli He, Samantha Iha, Erich Grotewold, Nan Jiang

Stem Cell

Development, Expansion, and Histological Characterization of Patient-Derived Liver Organoids for Drug Screening and Disease Modeling

Authors: Silvia De Siervi, Stefania Mantovani, Barbara Oliviero, Mario U. Mondelli, Martina Di Noia, Cristiana Soldani, Barbara Franceschini, Fabrizio De Luca, Marcello Maestri, Salvatore Corallo, Marco Lolicato, Stefania Cannito, Cristian Turato

We value your input and would love to hear your feedback on Bio-protocol.
Thank you for taking the time to share your thoughts with us!
Share Your Feedback

 

--

Bioclues is India's bioinformatics society working for bridging mentor mentee relationships.  Become a Life Member Toda and reap several benefits! 

https://bioclues.org/

Linkedin: http://www.linkedin.com/groups?gid=1339327

Facebook: https://www.facebook.com/groups/bioclues/

--

Prashanth N Suravajhala, Ph.D.

Professor, Systems Genomics Group

Department of Biosciences, Room # 323D, AB-3 

Manipal University Jaipur, Dehmi Kalan 303007,  India.

Founder, Bioclues.org

Group page: http://www.bioinformatics.org/wiki/Prash 

Twitter: @prashbio

"One rule is important in science- only courageous people win "   ~ Max Planck