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Latest Issue: May 20, 2026 Articles Published in May Articles in Press Archive

Call for Papers

Special Issue: Protocols for Organoid Research

We welcome submissions of step-by-step protocols and method-focused reviews addressing organoids, assembloids, organ-on-chip platforms, spatial omics, and related technologies and tools.

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Upcoming Webinars

May 27, 2026 11:00 AM ET | 8:00 AM PTRegister

Machine learning–assisted quantification of organelle abundance using Fiji

Organelle abundance is a key microscopic readout of organelle formation and, in many cases, function. Quantification of organelle abundance using confocal microscopy requires estimating their area based on the fluorescence...

Jun 18, 2026 11:00 AM ET | 8:00 AM PTRegister

Visualizing diverse RNA functions in living cells with the Spinach™ family of fluorogenic aptamers

RNA is now recognized as a highly diverse and dynamic class of molecules whose localization, processing, and turnover are central to cell function and disease. Live-cell RNA imaging is therefore essential for linking RNA behavior...

Bio-protocol: May 5 and May 20 Issues

Volume: 16, Issue: 10, May 20, 2026

Biochemistry

PEPTERGENT: A Peptide-Based Reagent for Detergent-Free Extraction of Membrane Proteins and Purification of Membrane Proteomes

Authors: Frank Antony, Ashim Bhattacharya, Franck Duong van Hoa

Bioinformatics and Computational Biology

A Step-by-Step GUI-Based Protocol for Molecular Dating Analysis Using PhyloSuite v2

Authors: Dong Zhao, Ivan Jakovlić, Xiantong Liu, Sishuo Wang, Dong Zhang, Tong Ye

Biophysics

Using Single-Particle Fluorescence Microscopy to Quantify Substrate Binding of Peptidoglycan-Modification Enzymes

Authors: Carlos Ramírez A. Carbó, Beiyan Nan

Cell Biology

Quantification of Spatial Patterns of Microtubule Transport by Kinesin-1 Head and Tail

Authors: Jashaswi Basu, Kajal Singh, Anita Jannasch, Chaitanya A. Athale

Isolation and Biophysical Characterization of Extracellular Vesicles Released by Myocytes

Authors: Kah Yong Goh, Wen Xing Lee, Hong-Wen Tang

Immunology

A Versatile In Vitro Quantitative Assay for Macrophage Efferocytosis in Diverse Research Applications

Authors: Xiaoge Xu, Tong Su, Qinuo Sun, Xiaoou Wang, Yanjie Liu, Huihui Wang

Microbiology

An In Vitro A-431 Epithelial Cell Infection Model for Studying Fungal Pathogenicity and Immune Responses Associated With Vulvovaginal Candidiasis

Authors: Victoria E. Groß, Kar On Cheng, Beatriz Cristóvão, Ezgi Gürel, Maximilian Himmel, Candela Fernández-Fernández, Jördis V. Schuchardt, Axel Dietschmann, Dolly E. Montaño, Mark S. Gresnigt

Plasmid Curing of Pseudoalteromonas haloplanktis TAC125 Using Homologous Recombination and PTasRNA Gene Silencing

Authors: Angelica Severino, Concetta Lauro, Marzia Calvanese, Ermenegilda Parrilli, Maria Luisa Tutino

Neuroscience

Chemoenzymatic Labeling Method for Detection of O-GlcNAcylated α-Synuclein Proteins by Western Blot

Authors: Hongrui Meng, Zhuoya Ma, Yongzhen Miao, Chun-Feng Liu

Plant Science

3D Reconstruction of Mature Arabidopsis Ovules Using FIB-SEM to Study Filiform Apparatus Morphology

Authors: Takao Oi, Tomoyo Morikawa, Yuna Yamazaki, Daisuke Maruyama, Daichi Susaki

Analysis of Cauline Leaf Development in Arabidopsis thaliana Using Time-Lapse Confocal Microscopy

Authors: Constance Le Gloanec

Biolayer Interferometry (BLI) to Quantify RALF1–Pectin Interactions

Authors: Susan Lauw, Elke Barbez

Correction

Correction Notice: Kinetic Determination of Cytochrome b₆f Activity In Vitro

Authors: Yuval Milrad, Daniel Wegemann, Michael Hippler

Volume: 16, Issue: 9, May 5, 2026

Biochemistry

A Suspension-Trapping Protocol for Bottom-Up Proteomics Sample Preparation

Authors: Joseph Schrader, Dennis Province, Nicholas A. DaSilva, Chang Liu

Reconstitution of Active Plant H⁺-ATPase AHA2 in Giant Unilamellar Vesicles

Authors: Huriye D. Uzun, Thomas Günther Pomorski

Biophysics

Lipid Analysis in Live Caenorhabditis elegans Using Solution-State NMR Spectroscopy

Authors: Florencia V. Guastaferri, Carla B. Delprato, Bruno Hernández Cravero, Gastón Prez, Diego de Mendoza, Andres Binolfi

Cell Biology

Assessing Mitochondrial Respiratory Complex-Associated Function From Previously Frozen Mouse Placental Tissue

Authors: Tina Podinic, Donald Xhuti, Cristina Monaco, Joshua P. Nederveen, Sandeep Raha

A Cell-Based Protocol to Assess Manganese Content and Relative Transport Activity of Manganese Transporters

Authors: Huiwei Zhong, Xurui Shen, Hanting Yang

Molecular Biology

Parallelised Cloning, Mammalian Cell Expression, and Purification of Nanobodies Identified by Phage Display

Authors: Hannah A. Campaigne, Kelly L. Parker, Raymond J. Owens, Lauren E-A. Eyssen

An Advanced Single-Cell RNA Sequencing (scRNA-seq) Protocol Utilizing Custom-Designed Multiplexing

Authors: Feng Gao, Xujie Liu, Fan Sun, Yadong Xiao, Gutian Xiao, Zhaoxia Qu

Neuroscience

Limited Proteolysis Mass Spectrometry to Identify Protein Structural Differences in Brain Tissue

Authors: Haley E. Tarbox, Stephen D. Fried

Plant Science

Kinetic Determination of Cytochrome b₆f Activity In Vitro

Authors: Yuval Milrad, Daniel Wegemann, Michael Hippler

TALENs and Related Technologies for Editing Nuclear and Organellar Genomes in a Model Plant, Arabidopsis thaliana

Authors: Chang Zhou, Shin-Ichi Arimura

Detecting Touch-Induced Calcium Dynamics With Live-Cell Imaging in Torenia Stigma

Authors: Xuan Zhou, Xiaofang Ma, Shuibo Yang, Shihao Su

Extraction and Isolation of Extracellular Vesicles From Piper betle Leaves Using the Apoplastic Fluid Washing and Size Exclusion Chromatography Method

Authors: Izy Sanddy, Wan Rahimatul Adawiyah, Der Jiun Ooi, Gayandi Ekanayake, Alireza Fazeli, Noor Izzah Abd Rahman, Norhayati Liaqat Ali Khan

Quantitative Assessment of Heat Shock-Induced Ferroptosis-Like Cell Death via Electrolyte Leakage in Arabidopsis thaliana Seedlings

Authors: Leah Kago-Gachao, Clara Cappa Salas, Myriam Sanchis Campos, Grace Whitmore, Daniela Sueldo

Stem Cell

Generation of Functional Patient-Specific Thymus Organoids From Human Pluripotent Stem Cells (hPSCs) Using Air–Liquid Interface Culture

Authors: Stephan A. Ramos, Holger A. Russ

Update

Update Notice: Immunopeptidomics Workflow for Isolation and LC-MS/MS Analysis of MHC Class I-Bound Peptides Under Hypoxic Conditions

Authors: Hala Estephan, Ester M. Hammond, Eleni Adamopoulou

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