LATEST RESEARCHCHROMOSOMES AND GENE EXPRESSION, DEVELOPMENTAL BIOLOGY Graham JM Hickey, Candice L Wike ... Bradley R Cairns Zebrafish early embryos initially package their developmental genes and enhancers in 'active' chromatin that subsequently receives polycomb repressive complex 1 (PRC1)-mediated histone H2A ubiquitylation, which confers silencing/poising – prior to Aebp2-PRC2-guided H3K27me3 addition. IMMUNOLOGY AND INFLAMMATION Sayumi Fujimori, Izumi Ohigashi ... Shinji Takada Highly specific gene manipulation in mouse thymic epithelial cells (TECs) shows that fine-tuning of β-catenin expression is required for TECs to generate an optimal thymic microenvironment to support T-cell development. EVOLUTIONARY BIOLOGY, NEUROSCIENCE Georg Welzel, Stefan Schuster An in silico analysis of gap junction proteins supports the hypothesis that connexins replaced the primordial innexins in chordate gap junctions due to an evolutionary bottleneck. NEUROSCIENCE Hui Zhang, Haifang Wang ... Jie He Single-cell transcriptomes of larval zebrafish whole brain shed light on the multidimensional landscapes of transcription factors and post-transcriptional regulators in vertebrate whole-brain neuronal classification and revealed principles of how neuronal cell diversity develops and evolves. ECOLOGY, EPIDEMIOLOGY AND GLOBAL HEALTH Marie C Russell, Catherine M Herzog ... Andrew C McCall While predators can clearly reduce mosquito populations by consumption, they can also have non-consumptive effects on mosquito body size and oviposition behavior, and these effects on vector traits can influence infectious disease dynamics. NEUROSCIENCE Aaron D Milstein, Yiding Li ... Sandro Romani Dendritic calcium spikes translocate hippocampal place fields by inducing a non-Hebbian form of bidirectional synaptic plasticity that operates over a seconds-long timescale called behavioral timescale synaptic plasticity. CELL BIOLOGY, MICROBIOLOGY AND INFECTIOUS DISEASE Jeffrey Y Lee, Peter AC Wing ... Ilan Davis Single-molecule analysis of SARS-CoV-2 RNA identifies significant heterogeneity in cellular viral RNA levels and highlights slower replication kinetics for the Alpha variant compared to the Victoria strain. IMMUNOLOGY AND INFLAMMATION Riem Gawish, Philipp Starkl ... Sylvia Knapp Only three Spike mutations enable murine SARS-CoV-2 infection, which is still strictly ACE2 dependent and causes a COVID-19-like disease in mice with immunopathology-driven lung damage. DEVELOPMENTAL BIOLOGY Merlin Lange, AhmetCan Solak ... Loïc Alain Royer ZAF (Zebrafish Automated Feeder) is the first fully automated, easy to build, and open source fish feeder designed for small and medium sized labs of which there are an estimated 3250 in 100 countries working with Zebrafish alone today. MEDICINE Xi Cao, Ting-Ting Shi ... Jin-Kui Yang A series of functional assays in mouse models and primary brown adipocytes confirm that the ACE2 pathway regulates glucose and lipid homeostasis, and maintains thermogenesis and systemic energy metabolism by both the Akt and PKA signaling. NEUROSCIENCE, STRUCTURAL BIOLOGY AND MOLECULAR BIOPHYSICS Johannes Elferich, Sarah Clark ... Eric Gouaux Direct imaging of individual molecules of the tip-link protein PCDH15 in mouse stereocilia shows that it assembles as a dimer, forms complexes that span stereocilia, and occurs in clusters with multiple copies of the PCDH15 dimer. IMMUNOLOGY AND INFLAMMATION, MICROBIOLOGY AND INFECTIOUS DISEASE Gemma Moncunill, Jason Carnes ... Raphael Gottardo Prevaccination levels of transcriptional modules related to monocytes, dendritic cells, and other immune categories correlate with clinical malaria risk in RTS,S/AS01E-vaccinated African children, suggesting that certain monocyte subsets may inhibit RTS,S/AS01E-induced protective responses. ECOLOGY, PLANT BIOLOGY Emanuela Cristiani, Anita Radini ... Dušan Borić A consistent pattern of consumption of Triticeae tribe grasses documented in the Danube Gorges of the Balkans since the Early Mesolithic might have facilitated a quick uptake of domesticated cereals due to a developed cultural taste and specific stone tool processing technology. PHYSICS OF LIVING SYSTEMS Philipp Fleig, Mirna Kramar ... Karen Alim Behavioural states emerge from variability in the complexity of underlying active contraction dynamics in a single-cell, network-shaped forager. NEUROSCIENCE David Dahmen, Moritz Layer ... Moritz Helias Short-ranged and random connectivity are sufficient to explain complex, long-range activity patterns observed in macaque motor cortex that are, moreover, flexibly adaptable to behavior. CELL BIOLOGY, IMMUNOLOGY AND INFLAMMATION Manuela Ecker, Richard Schregle ... Jeremie Rossy Sorting nexin 9, a membrane curvature inducing protein, creates a distinct environment within clusters of the co-receptor CD28 and thereby allows T cell activation to proceed to a successful outcome. DEVELOPMENTAL BIOLOGY, NEUROSCIENCE Sonia Taïb, Noël Lamandé ... Isabelle Brunet Angiogenesis and vascular development within peripheral nerve is regulated by Netrin-1, Schwann cells, and myelination. IMMUNOLOGY AND INFLAMMATION, MEDICINE Laura Amado-Rodríguez, Estefania Salgado del Riego ... Guillermo M Albaiceta Patients with different rs1990760 variants have a differential inflammatory response to severe SARS-CoV-2 infections, so that those with a TT genotype have an attenuated systemic inflammation and may not benefit from steroid therapy. NEUROSCIENCE Thomas Akam, Andy Lustig ... Mark E Walton pyControl is an open-source tool that makes it easy to specify complex behavioural tasks, run them at scale on low-cost hardware, and communicate task logic to other researchers. | 
