Bins from Binsanity-wf

[fernand...@gmail.com]

Binsanity-wf gives me, for example, such bins:

assembly-bin_0-refined_0.fna

assembly-bin_0-refined_1.fna

assembly-bin_1-refined_0.fna

assembly-bin_1-refined_1.fna

assembly-bin_1-refined_2.fna

Are there here 2 or 5 bins? In other words, are assembly-bin_0-refined_0.fna and assembly-bin_0-refined_1.fna still a single bin?

Thanks,

[edgraham]

Hello,

So this means there are 5 bins.

To explain what those mean you have to think about how the Binsanity-wf script works. So Binsanity-wf first clusters all your contigs based solely on coverage which generates an initial group of bins (in this case assembly-bin_0 and assembly-bin_1). These bins are then evaluated with CheckM and refined now using the tetranucleotide frequencies and GC content. This is where the 'refined' comes from. So following refinement of assembly-bin_1 you now have 3 bins: assembly-bin_1-refined_0, assembly-bin_1-refined_1, and assembly-bin_1-refined-2. 

I can make a note to change the naming schema so that it is less confusing in the future. 

Regards,

Elaina