New things in BinSanity v0.2.5.11

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edgraham

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Aug 9, 2017, 4:51:33 PM8/9/17
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Hi All,

I just wanted to give an update on the changes for v0.2.5.11 of BinSanity

  • Binsanity-wf and Binsanity-lc scripts have been changed to enable the user to specify a prefix to append to all files and directories generated throughout the process of clustering to enable people to run Binsanity in parallel if they'd like to test multiple sets of parameters. 
  • I added a new script to the utilities section of BinSanity because why not. This is the `concat` script which can be used to concatenate protein alignments when building a phylogenetic tree. To use it you can call it as such:
    • concat -f directory/where/alignments/are -e extension/for/alignment/in/fasta/format --Prefix [Prefix That Links Alignments] -o Output File -N [Minimimum Number of Sequences to be in concatenation]
    • In this the -N is used to specify how many of the proteins you are concatenating that any single organisms needs to have to be included in the final concatenated fasta file. So for example if you have 16 ribosomal markers you may say that any organism in your tree needs to have at minimum 13 of the 16 proteins to be included.
  • If you haven't already, check out our preprint for the clustering of 2,631 genomes using Binsanity on the Tara oceans dataset. We have also provided a protocolsIO for the whole procedure!

As always contact me with any suggestions or questions about using BinSanity.

Thanks,
Elaina
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