haplotype analysis

[ibrahim acikbas]

Hello I need a suggestion for analyzing my rflp data. I know all have sequencing data but I do not sequencing facility and budget. It may more basic for you but I am newbe.   I have PCR-RFLP data for un related 100case and 100 control subjects. I do not have parental genotypes.   I analyzed four polymorphism by four restriction endonuclease within the same gene in individuals with a particular disease and control subjects.   Like this: If BamH1 cuts = allel is B       AvaI  cut = A If BanH1 uncuts = allel is b     AvaI uncut = a   case 1 = Bb mm Aa Hh             control1 = Bb Mm AA Hh case 2 = bb  mm Aa Hh             control2 = Bb mm Aa Hh   I have alleles and genotypes for each polymorphism   I want to test an association between the genoypes and the studied disease and most importantly  test an association between haplotypes and the studied disease.   the genotypes / haplotypes that I obtained is phased or unphased?   How can I assign / infer a haplotype?   How can I test the association between haplotype and the disease?   What software should I use? How can I interpret the result file?   sincerely   Ibrahim