I first ran sparse_pregraph like so:
SOAPdenovo-127mer sparse_pregraph -s hsapiens.config -K127 -z5000000000 -R -o hsapiens -p32
It completed successfully.
Total number of marks in file markOnEdge: 1103907620
Time spent on building preArcs: 6234s.
Overall time spent on constructing lightgraph: 370m.
I then ran contig, and it segfaults immediately:
SOAPdenovo-127mer contig -g hsapiens -R -p32
Version 2.04: released on July 13th, 2012
Compile Jun 3 2016 23:46:00
********************
Contig
********************
Parameters: contig -g hsapiens -R -p 32
There are 5962126 kmer(s) in vertex file.
There are 17647475 edge(s) in edge file.
make: *** [Makefile:25: hsapiens.contig] Segmentation fault (core dumped)
Any suggestions as to what's going on here? I'm going to try pregraph next rather than sparse_pregraph. See below for the config file.
Thanks,
Shaun
$ cat hsapiens.config
max_rd_len=250
[LIB]
avg_ins=400
asm_flags=3
rank=1
pair_num_cutoff=3
map_len=32
p=pe400.fa.gz
[LIB]
avg_ins=6000
reverse_seq=1
asm_flags=2
rank=2
pair_num_cutoff=5
map_len=35
p=mp6k.fa.gz