9/11 STR Analysis

[kimberly...@comcast.net]

Hi All,

I'm looking at pg. 130 with the 9/11 STR Analysis.  I have the 4 profiles in order and have labeled them from the top:  mom, dad, son, and son.  But, I'm at a loss as to how to explain the data.  Was it that the mom or dad's dna was recovered and the profiles were compared to see if he/she was a match to the family?  Thanks for any help,

Kim

[Patti Bertino]

I think you are referring to page 130 in the binder correct?   This was the DNA analysis using STR  Short Tandem Repeats. It was an actual printout from a student whose father was missing after 911. The student brought the analysis to school and asked the teacher to help him understand the DNA analysis.   Top line: mother Second line: Remains recovered from 911   They are trying to determine if these remains could be this woman's husband and the father of her two children, two boys   Third and Fourth LIne: DNA from the two sons.   #1 First identify the sex of each of the DNA printouts       Note that only the top line has XX, all others are XY (fifth probe from the left)   #2 The numbers refer to the number of short tandem repeats for each of the probes used in the analysis.         Note that a total of 13 probes were used.   #3 First probe (data to far left)     Mom has a repeat of 14 on one gene and a repeat of 18 on the other gene     (remind students that you usually get one gene from one parent and one gene from the other parent, you don't get both of their genes (or in this case you don't get both of the mother's STR; one or the other)       Father (or suspected father from his remains) has repeats of 15 and 16   #4 Third line: Son has a repeat of 15, 18         The 18 came from the mother and the 15 from the father   #5 Fourth line  Son has a repeat of 14 from the mother and 15 from the father   Continue checking the DNA and number of repeats for all 13 probes. You want to determine if the DNA recovered (line 2) could have been these two boys father and this woman's husband.   Note on the second probe in the father's DNA you only see one number 12. That is because for that gene the man was homozygous: he got a repeat of 12 from one parent and a repeat of 12 from the other parent.   If you examine all 13 probes, you will find that it appears that this missing DNA could be the father of the two boys and the mother.   I find it helpful to have students circle the STR from the mother in pink and the STR inherited from the father in blue.   After checking all 13 probes, you could then get into probability and statistics. How common is a STR of a certain number.   We covered this STR Analysis while others in the group did the actual DNA gel electrophoresis.   I would suggest having students research  STRs before doing this STR analysis.   Patti Nolan Bertino          -------Original Message-------

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[Kim Boardman]

Perfect thank you so much! 

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