Mario was born in the Mushroom Kingdom. Soon, he and Luigi would save the day from Bowser on a regular basis. They moved to the Real World because Mario grew tired of saving Peach from Bowser and going through his castles all the time. He is the leader of the Mushroom Force. He and Wario both hold the title of Chosen One, originally thought by Merlin to be possessed by Arthur. Mario is, along with his younger brother Luigi, a former plumber, superhero, and former resident of the Mushroom Kingdom.
In the Real World, Mario and Luigi spend their time relaxing and having fun. Bowser promptly hired his henchman Wario (and eventually, Waluigi) to bring the Mario Brothers back to the Mushroom Kingdom with them by force. However, the duo refused to return and continued their ways of having fun in the Real World. Mario would later become the lead singer of Johnny Mushroom and the Kingdoms and make several best friends along the way.
On a family vacation, their plane was blown up by Kamek on King Bowser and Nox Decious' orders. Thankfully, Merlin was able to rescue the infants, but unfortunately, he was unable to save their parents.
Merlin teleported the infants to Yoshi's Island, where Yoshi found them and brought them back to him. Merlin then gifted the two brothers with their signature hats, and had them raised in the Real World.
After saving the day many times, the Mario Brothers decided to take a well-deserved vacation in the Real World. They managed to purchase a home and began to relax and have fun; however, they were completely unaware that Merlin, the one who raised them, was always keeping a watchful eye on them.
Mario and Luigi receive a letter from King Bowser, who declares he has kidnapped Peach and taken over the Mushroom Kingdom once again. However, the two brothers refuse to return and continue taking a vacation in the Real World, irritated with always having to save Peach and defeat Bowser.
Throughout the season, they are continuously being bothered by Bowser's letters and Wario's attempts to return them to the Mushroom Kingdom. After Mario gets annoyed once again by a letter from Bowser (who tells him and Luigi that he has kidnapped Peach again and that they cannot stop him), Mario decides to go look for some super mushrooms with Luigi rather than saving her, thus ignoring the letter. After finding some, they are rudely interrupted by Wario, who had been sent by Bowser to take them back to the Mushroom Kingdom. Mario refuses, saying that he is sick of saving Peach as he briefly tugs at Luigi by his overalls in frustration. He tells Wario that he can inform Bowser that he and Luigi are not coming and Luigi agrees, saying, "Yeah! What Mario said! Hmph!". Due to the cold, Wario tells the brothers to come inside with him to talk the matter over. In Wario's house, Wario orders Mario and Luigi to come with him to the Mushroom Kingdom. Mario tells Wario that he is tired of his enemies constantly interrupting his vacations and trying to mess it up for their own amusement and asks Wario to get Link to rescue Peach for a change. But Luigi tells Mario that Link has his own problems, but Mario doesn't care and makes it clear that he and Luigi are staying in the Real World. Wario tells them that Bowser figured they would say that, so he had told Wario to fight them just in case. Unfazed, Mario wishes Wario good luck since he and Luigi just beat Sonic in the Olympic Games, to which Luigi says, "Yeah! What Mario said! Again!". Wario laughs and says that Sonic is past his prime and is slower than a "mangled Goomba", so the brothers' beating him wouldn't count or mean anything. Offened, Mario calls Wario a "son of a Goomba" and says he will never take them alive. He manages to distract him by pointing at nothing and asking, "What's that over there?!" and as Wario turns to look, Mario and Luigi immediately get up and escape while screaming. This makes an enraged Wario promise to return to get them someday as he lets out an evil laugh. ("Tired of the Mushroom Kingdom")
After receiving another letter from Bowser (who is starting to get bored without them), Mario and Luigi go home to play Wii Tennis on the couch. However, they get into an argument after Mario insults Luigi's Wii Tennis abilities. Fed up with Mario's arrogance, Luigi leaves and joins up with Wario. During this time, Mario starts to miss him and soon regrets his rudeness toward him earlier. ("A Tale of Two Brothers")
After Wario gets tired of Luigi being around, he convinces him to go back to Mario. Once the brothers receive a third letter from Bowser, they proceed to watch the Lord of the Rings trilogy. During the films, Mario doesn't stop talking and crying, inadvertently ruining Luigi's first experience with the films. Then Mario cruelly jokes about Aragorn's death since Luigi was unable to tell what happened and then wonders what Wario watches. ("One Ring that is better than a Compass")
After cleaning out Donkey Kong's sink after he urinates in it, Mario talks about how horrible of a roommate Donkey is with Luigi. Then they decide to go play soccer. Mario easily wins the soccer match and gloats in victory until Luigi points out that Wario and another person is approaching them. It is Wario with his younger brother and new henchman and sidekick Waluigi, whom Wario introduces the Mario Brothers to. ("Wario's New Partner likes Purple")
Mario and Luigi begin their first battle with Wario and Waluigi. After a long battle, Mario finally manages to defeat them with two massive fireballs. Mario tells them they are going to stay in the Real World while Wario and Waluigi refuse and leave. After the battle, Mario decides to go get a pizza with Luigi. ("Make Fireballs, Not Love!")
After receiving yet another letter from King Bowser, Mario and Luigi decide to grow their mustaches back, but shave them off soon after. Then Mario and Luigi decide to ride their go-kart, which is later stopped when a ball falls in front of it. Mario and Luigi find someone with a hat hiding behind a tree. After dancing around to the Pokmon theme song, he introduces himself as Ash Ketchum, who thought that Mario and Luigi were rare Pokmon and tried to catch them in his Pokball. Mario tells Ash to leave and stop bothering them, forever remembering Ash as the one he hates most. ("Why catch them All?")
Afterwards, Mario and Luigi decide to form a rock band, which they hold auditions for. They turn down Ash when he tries to play the Pokflute, but Wario and Waluigi are accepted. Mario does lead vocals and guitar, Luigi plays rock band drums, Wario plays guitar, and Waluigi plays keyboard. After rehearsing and parodying several songs, Mario ends the band and leaves due to extreme boredom. ("A Band that is better than the Jonas Brothers!")
Later, Mario and Luigi hang out at the mailbox when Ash walks up to get his mail. He waves at them and they wave back; however, when he turns his head, they show disgust. After reading a letter from Pikachu, they are asked by Ash if they would plunge his toilet with promises of pizzas, sodas, etc. Mario agrees and the trio proceed to randomly dance their way to Ash's house to "Let's Groove" by Earth, Wind & Fire. After arriving, Mario wonders how to plunge since he and Luigi haven't done it for many years. After numerous attempts, the Mario Brothers finally get Wobbuffet out of the toilet, but decide to quit their jobs as plumbers due to the hassle. They ask Ash for their pizza when he reveals that he lied to them about it. Angered with Ash, Mario and Luigi storm out and begin to find new jobs. ("It came from Ash's Toilet")
Mario and Luigi begin their search for new jobs, starting with a printer store. The owner is borderline insane, but they still want the job. Their request is shot down when they discover Wario and Waluigi already have the job and they tease the Mario Brothers about it. As they leave, the Dance Critic asks the two to dance for her and pushes them a short distance away from her so they can do so. After dancing to "Apache (Jump on It)" by the Sugarhill Gang, they are immediately shooed away by the Dance Critic since she did not like their dancing skills after all. They go to RMA Games, where they speak with Bob, Joe, Terence, and Phillip to become game designers and pitch their idea of Super Mario Brothers. Unfortunately, they are once again turned down and shunned. In the end, Mario and Luigi do not get a job, so they decide to go back to the way things were. ("Real Jobs Suck")
Afterwards, Mario and Luigi get some bananas from the RMA Fruit Stand, but they are chased by Donkey Kong since he believes they stole his bananas after receiving a letter from Diddy Kong stating that two plumbers stole the bananas and that Donkey has to find and retrieve them immediately.
When Donkey Kong accuses Mario and Luigi of stealing his bananas and tells them to prepare to face his "DK wrath", a scared Luigi tells him they got the bananas at the RMA Fruit Stand and didn't steal Donkey Kong's at all. Unfortunately, he doesn't believe them, convinced that they are lying, and prepares to chase them. Mario and Luigi scream in terror as they throw away their bananas and run away from the infuriated gorilla while flailing their arms.
Sooner or later, Mario and Luigi hide behind the front of a car and remain there until Donkey Kong appears to have lost them. However, before they can get out of their hiding spot, Donkey appears out of nowhere and scares Mario and Luigi, who proceed to run away from the gorilla once again.
Eventually, Donkey manages to corner Mario and Luigi and prepares to kill them while screeching as they both scream in fear. However, before he can do so, Mario, Luigi, and Donkey Kong notice the UPS man arriving with another letter. The letter turns out to once again be from Diddy Kong, who tells Donkey that Wario and Waluigi are really responsible for stealing the bananas, adds not to chase Mario and Luigi around as it would be stupid, and bids Donkey Kong farewell. Turning to the Mario Brothers, Donkey tries to apologize for chasing them while admitting that he was wrong to think they stole his bananas, but Mario angrily refuses, orders the gorilla to get out of his face and leaves with Luigi (who agrees with his brother and leaves with him), thus making Donkey Kong feel bad as he hangs his head in sadness and sighs. ("A Monkey for your Thoughts?")
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