Hello all,
I am running a 16S alignment with 190 sequences, and I ran 6 independent runs: 2 with 35 million chain length and 4 with 60 million. All of my ESS values are high except for the 6 rate parameters (rateAC, rateAG, etc.). In my outputted MCC tree, the posterior probabilities were very variable, with many over 0.9 but several below 0.1. Are the low rate ESSs contributing to this? And if so, what can I do to improve them?
Thanks