BA900 - Bay Area 900 at UC Berkeley - Availability
Jeffrey G Pelton
This message is to remind you of the
availability of the Bay Area 900 MHz
system housed at UC Berkeley. Currently,
there are no time charges for use of
the instrument. The system is equipped
with a cryoprobe optimized for both 1H
and 13C detection. The latter makes it possible
to do 2D expts with detection on 13C.
The system and cryoprobe have been performing
well. Recently, Andreas Lingel (in Wayne
Fairbrother's group at Genentech) successfully
recorded a 2D 1H-15N TROSY spectrum of a 95 kDa
complex in which one of three proteins was
15N-13C-2H labeled and the other two were unlabeled.
Click on the following link to view the data:
http://www.cchem.berkeley.edu/wemmer/BayArea900/page15.html
Seth Rubin for UC Santa Cruz has also collected
interesting data on a peptide-protein complex.
Based on the work of the Wagner group (Harvard), we have recorded
several spectra using nonlinear sampling techniques. Below (attached)
are 2D planes from 3D TROSY HNCACB spectra recorded with linear
and nonlinear sampling. The nonlinearly sampled data show
much better resolution, although weaker peaks tend to be missing.
We have more work to do in this area, but would be happy to
share what we have learned so far. Thanks to Kaya Erbil of the
Wemmer group for getting initial nonlinear sampling / processing
experiments up and running.
To request instrument time, please contact me at jgpe...@berkeley.edu
or call me at 510-666-2752.
To view the homepage and link to the calendar, see the following:
http://www.cchem.berkeley.edu/wemmer/BayArea900/BayArea900.html
Sincerely,
Jeff Pelton
--
Jeffrey G. Pelton, Ph.D.
jgpe...@berkeley.edu
510-666-2752 - office
510-666-2753 - NMR Room
Central California 900 MHz NMR Facility at UC Berkeley
http://www.cchem.berkeley.edu/wemmer/BayArea900/BayArea900.html
