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We are pleased to announce
the immediate availability of a new release of AUTO3DEM.
In addition to minor bug fixes, there are few changes that are
noteworthy:- the license ha been changed to follow the
recommendations of the University. Essentially the software is
free to use, modify and distribute for research purposes;
- better support of large maps (>1200 pixels along each size);
- the program auto3dem now enforces a resolution-limited approach
when automatically determining the resolution range to use for
determining the particle parameters, in order to minimize noise
overfitting;
- auto3dem: added option for automatically determining, at each
iteration, the optimum radial range to use for masking the
reference map (auto hollow_auto). To use in combination with 'auto
hollow_map' set to 1;
- auto3dem: provide by default an optimum radial range for
template masking at the end of the Random Model Computation;
- added new program, flatten, to automatically remove background
density from the reference map, and added an option in auto3dem
(auto flatten_map) to set to 1 in order to use it.
- the metrics for determining the alignment in PO2R have been
optimized and now they all report their values as phase residuals,
in degrees (soon an exact formulation for all of them will be
provided);
- the first line of the parameter (i.e. dat) files can now
include, after the name of the stack, 3 fields describing the box
size of the particles (along x and y) and the number of particles.
These values are optional but most of the programs add them, in
order to minimize the access to the stack file;
- auto3dem: changed behavior of ‘auto freeze_res’ in order to only
freeze the range of resolution frequencies used for determining
particle parameters, but to allow the reconstruction to be
performed according to the resolution estimated;
- the routine for CTF refinement has been improved and the way it
is controlled by auto3dem has changed. Another email will follow
shortly with some information about it.
You can look at the modification history (file
MINOR_RELEASE_NOTES) for a list of all the changes.
Sincerely,
Giovanni
PS: this is a second attempt to send this email. If already
received, please disregard.