EPUB & PDF Ebook CRISPR-Cas: A Laboratory Manual | EBOOK ONLINE DOWNLOAD
by by Jennifer Doudna (Editor), Prashant Mali (Editor).
Ebook EPUB CRISPR-Cas: A Laboratory Manual | EBOOK ONLINE DOWNLOAD
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Description
The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before. Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system--especially for minimizing off-target effects--are also provided. Authors also describe other applications of the CRISPR-Cas system, including its use for regulating genome activation and repression, and discuss the development of next-generation CRISPR-Cas tools. The book is thus an essential laboratory resource for all cell, molecular, and developmental biologists, as well as biochemists, geneticists, and all who seek to expand their biotechnology toolkits.
Let's be real: 2020 has been a nightmare. Between the political unrest and novel coronavirus (COVID-19) pandemic, it's difficult to look back on the year and find something, anything, that was a potential bright spot in an otherwise turbulent trip around the sun. Luckily, there were a few bright spots: namely, some of the excellent works of military history and analysis, fiction and non-fiction, novels and graphic novels that we've absorbed over the last year.
Here's a brief list of some of the best books we read here at Task & Purpose in the last year. Have a recommendation of your own? Send an email to ja...@taskandpurpose.Com and we'll include it in a future story.
Missionaries by Phil Klay
I loved Phil Klay’s first book, Redeployment (which won the National Book Award), so Missionaries was high on my list of must-reads when it came out in October. It took Klay six years to research and write the book, which follows four characters in Colombia who come together in the shadow of our post-9/11 wars. As Klay’s prophetic novel shows, the machinery of technology, drones, and targeted killings that was built on the Middle East battlefield will continue to grow in far-flung lands that rarely garner headlines. [Buy]
- Paul Szoldra, editor-in-chief
Battle Born: Lapis Lazuli by Max Uriarte
Written by 'Terminal Lance' creator Maximilian Uriarte, this full-length graphic novel follows a Marine infantry squad on a bloody odyssey through the mountain reaches of northern Afghanistan. The full-color comic is basically 'Conan the Barbarian' in MARPAT. [Buy]