Hi, I am using a typical ucsc gtf file downloaded from the ucsc genome browser, btw getting an error for invalid file format in gtf file looked at the test file, my file looks ok.Pls see below
#name chrom strand txStart txEnd cdsStart cdsEnd exonCount exonStarts exonEnds proteinID alignID
uc007aet.1 chr1 - 3195984 3205713 3195984 3195984 2 3195984,3203519, 3197398,3205713, uc007aet.1
uc007aeu.1 chr1 - 3204562 3661579 3206102 3661429 3 3204562,3411782,3660632, 3207049,3411982,3661579, Q5GH67 uc007aeu.1
uc007aev.1 chr1 - 3638391 3648985 3638391 3638391 2 3638391,3648927, 3640590,3648985, uc007aev.1
uc007aew.1 chr1 - 4280926 4399322 4283061 4399268 4 4280926,4341990,4342282,4399250, 4283093,4342162,4342918,4399322, NP_001182591 uc007aew.1
uc007aex.2 chr1 - 4333587 4350395 4334680 4342906 4 4333587,4341990,4342282,4350280, 4340172,4342162,4342918,4350395, Q548Q8 uc007aex.2