The Binding Of Isaac Extra Hud
Edco Haglund
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The role of the telomere repeat-binding factor 2 (TRF2) in telomere maintenance is well-established. However, recent findings suggest that TRF2 also functions outside telomeres, but relatively little is known about this function. Herein, using genome-wide ChIP-Seq assays of TRF2-bound chromatin from HT1080 fibrosarcoma cells, we identified thousands of TRF2-binding sites within the extra-telomeric genome. In light of this observation, we asked how TRF2 occupancy is organized within the genome. Interestingly, we found that extra-telomeric TRF2 sites throughout the genome are enriched in potential G-quadruplex-forming DNA sequences. Furthermore, we validated TRF2 occupancy at several promoter G-quadruplex motifs, which did adopt quadruplex forms in solution. TRF2 binding altered expression and the epigenetic state of several target promoters, indicated by histone modifications resulting in transcriptional repression of eight of nine genes investigated here. Furthermore, TRF2 occupancy and target gene expression were also sensitive to the well-known intracellular G-quadruplex-binding ligand 360A. Together, these results reveal an extensive genome-wide association of TRF2 outside telomeres and that it regulates gene expression in a G-quadruplex-dependent fashion.
Extra-illustrated books provide rich and often surprising pictorial sources for their respective subjects. They differ from scrapbooks in that the themes of the material added by the collector are dictated by the texts that form their backbone. Examples can be traced to the early 15th-century (the Huntington Gutenberg Bible once had religious prints mounted inside its covers). The earliest examples, some of which were manuscripts, are Continental.
Extra-illustrating a book usually involved mounting the new material, and often every leaf of the book, in paper frames of uniform size. The expanded work was then newly bound in the sumptuous style favored by collectors of the age. Because of the value of the contents and the price of inlaying and binding, it was an expensive hobby, and Grangerized books fetched high prices on the antiquarian book market.
Though they are fascinating in their own right as evidence of the collecting culture and social networks associated with this strange hobby, the greatest value of extra-illustrated books for most researchers lies in their rare prints and other added material. They are a major visual and historical resource; more than 90 percent of intaglio prints at the Huntington reside not in the Art Museum, but in the library, between the covers of Grangerized books. Yet because of the immense labor required to catalog them in detail, their contents are still little known. A dip into one of these books can be like a treasure-dive in uncharted waters.
A monograph on privately illustrated books: a plea for bibliomania, by Daniel M. Tredwell
Lincoln Road, Flatbush, L.I.: Privately Printed [New York] : [The DeVinne Press], 1892.
Call number: Z1023 .T72
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Feature papers represent the most advanced research with significant potential for high impact in the field. A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications.
Abstract: Allosteric modulators have emerged with many potential pharmacological advantages as they do not compete the binding of agonist or antagonist to the orthosteric sites but ultimately affect downstream signaling. To identify allosteric modulators targeting an extra-helical binding site of the glucagon-like peptide-1 receptor (GLP-1R) within the membrane environment, the following two computational approaches were applied: structure-based virtual screening with consideration of lipid contacts and ligand-based virtual screening with the maintenance of specific allosteric pocket residue interactions. Verified by radiolabeled ligand binding and cAMP accumulation experiments, two negative allosteric modulators and seven positive allosteric modulators were discovered using structure-based and ligand-based virtual screening methods, respectively. The computational approach presented here could possibly be used to discover allosteric modulators of other G protein-coupled receptors. Keywords: GLP-1R; virtual screening; allosteric modulator; drug discovery; molecular docking
Zhou, Qingtong, Wanjing Guo, Antao Dai, Xiaoqing Cai, Mrton Vass, Chris de Graaf, Wenqing Shui, Suwen Zhao, Dehua Yang, and Ming-Wei Wang. 2021. "Discovery of Novel Allosteric Modulators Targeting an Extra-Helical Binding Site of GLP-1R Using Structure- and Ligand-Based Virtual Screening" Biomolecules 11, no. 7: 929.
For over a century, Hellmann's Extra Heavy Mayonnaise has been delighting taste buds with its creamy, rich flavor thickened with extra egg yolks. This extra curdy, viscous texture makes the mayo ideal for binding and browning, enhancing your dishes with a perfect balance of flavor. Hellmann's Extra Heavy Mayonnaise is made with real ingredients like 100% cage-free eggs, oil, and vinegar for a rich, creamy flavor your guests can savor. Ideal for cafs, restaurants, and catering services, this delicious mayo sauce excels as both a condiment, dressing, and ingredient. Its versatility shines through various culinary applications, sandwiches and burgers, to dips and dressings, or to aid in attractive browning. Superior emulsion gives the mayonnaise outstanding stability, making it ideal for dressings, bound salads, and dips. With Hellmann's gluten free and kosher formula, you can easily accommodate the dietary requirements of your customers without sacrificing taste or quality.
A foodservice exclusive! Hellmann's Extra Heavy Mayonnaise is made by adding extra egg yolks to our trade-secret Real Mayonnaise recipe. This thicker, more eggy mayonnaise inspires passion and loyalty among professional chefs. Thicker, extra-curdy viscosity holds through demanding binding and browning. The thicker emulsion is an ideal dressing base for extra-creamy scratch-made dressings. A chef exclusive! We know to really "Bring Out The Best" for your guests we need to do more than just taste great! Hellmann's Extra Heavy Mayonnaise is made by adding extra egg yolks to our trade-secret Real Mayonnaise recipe. This thicker, more eggy mayonnaise inspires passion and loyalty among profession
How do you determine how much batting you need for a square quilt, i.e. 88x88 inches?
ReplyI like to have an extra 3-4 inches around each outside edges of my quilt top when I quilt on my Viking D1 sewing machine.
That means for an 88 inch square quilt, I'd add 6 to 8 inches and cut my quilt batting somewhere between 94 to 96 inches square.
This extra batting accommodates any drawing up of the quilt as it is stitched and allows for squaring up before binding.
If you were quilting a small wall hanging or table runner, you could get by adding as little as an inch or two to each side (or 2 to 4 inches to the length and the width measurements).
Handling this extra batting...Particularly if you use a cotton or bamboo batting, the bat can 'shed' fibers onto your quilt top.
Once you've finished your stabilizing quilting, you can freely move around your quilt to quilt different areas.
In this quilt just below, I ditch quilted between all the blocks and sashing. I then free motion ditch quilted the seams of the center star (not shown) and all the little stars. Then I quilted the outside flying geese borders. The alternate plain squares will all be free motion quilted with wreaths, so there's lots more stitching to be done. I'll want to protect the edges and here's how.
The extra quilt batting is trimmed
In the top photo, a lot of the extra batting was cut off once the borders were quilted. Most, but not all, because I'll block and square up this quilt once the quilting is finished.
The extra quilt batting is wrappedTo keep the edges neat I wrap the excess backing fabric around to the front and safety pin it in place to protect the edges. If I had used a cotton batting, this would also keep the fibers from shedding the sandwich is moved back and forth under the needle.Using the services of a long arm quilter...If you choose to send your quilt out to a long arm quilter, they may want a larger square, requiring as much as 4-6 inches of extra batting on each side. This is because the batting is held on the machine by rolling it onto a bar/roller. You would need to check with the quilter for their specific requirements.
There are a couple of other pages on my website about quilt batting and layering and basting in general. They are:
- Layering and Basting a Quilt
- Machine Quilting a Beginner Quilting Project
Thank you for your question. I hope this information has been helpful to you!
Piecefully,
Julie Baird
Editor
The influence of intracellular and extracellular protein binding on the hepatic storage and biliary elimination of dibromosulfophthalein (DBSP) was studied in isolated perfused rat liver. Under first order kinetic conditions the amount of DBSP in the liver at a given plasma concentration (hepatic storage) was determined by extracellular binding to albumin and intracellular binding to the cytosolic Y and Z proteins as well as concentrative membrane transport from plasma into the liver. At higher doses, extensive binding of DBSP to intracellular organelles also occurred while liver cytosol/plasma concentration gradients of unbound DBSP were much lower. Hepatic storage increased with decreasing albumin concentration in the perfusate of isolated perfused rat livers. However, it was shown that this parameter is dose-dependent, and errors can be introduced in its calculation if nonlinearity of sinusoidal and canalicular transport processes as well as nonlinear protein binding are not taken into account. The influence of another organic anion, indocyanine green (ICG) on the hepatic storage, subcellular distribution, and elimination of DBSP was subsequently studied. At equimolar amounts the presence of ICG resulted in a 50% decrease in hepatic clearance and hepatic distribution volume of DBSP. It was inferred that these changes are due to an inhibition of carrier-mediated transport across the sinusoidal and canalicular membrane and preferential displacement from intracellular binding sites. In contrast DBSP in equimolar amount enhanced the initial disappearance rate and biliary excretion of ICG, probably due to increasing its free fraction in plasma. It is concluded that the level and mechanism of interaction of two drugs within the eliminating organ can be characterized by combining clearance studies with data on subcellular and extracellular binding.
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