If your input file is too big then you can cut off it. Here are some help to do that:
1. Use head and tail commands in a Linux terminal:$ head -150000 input.fa > out1.fa
$ tail -150000 input.fa > out2.fa
These command line create two new files with the content of input.fa from line #1 to #150000 and from line #150001 to line #300000.
2. Use VIM editor:
$ vim input.fa
:1,150000 w out1.fa
:150001,300000 w out2.fa
These command line create two new files with the content of input.fa from line #1 to #150000 and from line #150001 to line #300000.
3. Multiple fasta/fastq file splitter in BiopythonUse this Biopython program:
Split large file