Dear Sir or Madam,
I hit error messages where no groups or files are found or if I do differently a similar error message.
In the first window of the AltAnalyze I call my experiment "RNASEQANA" I then designate the location of my four fastq files. Finally, I designate the output folder location.
As per instructions, into "Expression input" folder I created a file called "groups.RNASEQANA" i.e, "groups" followed by the name of my experiment and still didn't work, I then did the same but added the individual fastq file names after "RNASEQANA" shown below and still didn't work.
Is there some specific way to create the group files that I am doing incorrectly? Does it need to be an excel file? e.g., ending in .xls? Am I using the correct expression input folder location to enter the groups? Finally, is there an easier way to assign groups to my fastq files? and also comparisons because it is likely when I get this to work finally it will error because no comparisons are assigned.
I attached a ppt file with screen shots of my workflow and the error messages. I tried several ways to get my groups assigned as you will see in powerpoint screen shots.
I am running windows 10 home on 64 bit operating system
My fastq files names as follows:
1) "CRD038401_1" (read 1)
2) "CRD038404_1" (read 2)
3) "CRD038407_1" (read 2)
4) "CRD038415_1" (read 1)
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Children's Hospital of Philadelphia