Dear Altanlyze team,
I ran Altanylze on RNA SEQ FASTQ files from some control samples.
After analysis, I am confused about what these values actually mean.
Could you help me understand what the values in the expression input file mean
In the EXP file, If gene A of sample 1 has a value of 182 what does that value mean?
What doe the values in the counts file mean? If gene A of sample 1 has 5,300 counts what does that mean? What are isocounts?
What do the valules in the transcript file mean?
In expression output file:
The same gene A in sample 1 when I open DATASET file has a different value than that shown in EXP file.
Also, why are the values in AVERAGE file the same as those in the EXP file?
Gene expression in control samples:
Finally, If not comparing to anything an I just want to know how much something is expressed in control samples is there an appropriate way to run the program.
Any help is very much appreciated.