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Bacterial amyloids
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asfyso
Apr 28, 2012, 8:03:33 AM4/28/12
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Methods Mol Biol. 2012;849:303-20.
Bacterial amyloids.
Zhou Y, Blanco LP, Smith DR, Chapman MR.
Source
Department of Molecular, Cellular, and Developmental Biology,
University of Michigan, Ann Arbor, MI, USA.
Abstract
Many bacteria can assemble functional amyloid fibers on their cell
surface. The majority of bacterial amyloids contribute to biofilm or
other community behaviors where cells interact with a surface or with
another cell. Bacterial amyloids, like all functional amyloids, share
structural and biochemical properties with disease-associated
eukaryotic amyloids. The general ability of amyloids to bind amyloid-
specific dyes, such as Congo red, and their resistance to denaturation
have provided useful tools for scoring and quantifying bacterial
amyloid formation. Here, we present basic approaches to study
bacterial amyloids by focusing on the well-studied curli amyloid
fibers expressed by Enterobacteriaceae. These methods exploit the
specific tinctorial and biophysical properties of amyloids. The
methods described here are straightforward and can be easily applied
by any modern molecular biology lab for the study of other bacterial
amyloids.
Bacterial amyloids.
Zhou Y, Blanco LP, Smith DR, Chapman MR.
Source
Department of Molecular, Cellular, and Developmental Biology,
University of Michigan, Ann Arbor, MI, USA.
Abstract
Many bacteria can assemble functional amyloid fibers on their cell
surface. The majority of bacterial amyloids contribute to biofilm or
other community behaviors where cells interact with a surface or with
another cell. Bacterial amyloids, like all functional amyloids, share
structural and biochemical properties with disease-associated
eukaryotic amyloids. The general ability of amyloids to bind amyloid-
specific dyes, such as Congo red, and their resistance to denaturation
have provided useful tools for scoring and quantifying bacterial
amyloid formation. Here, we present basic approaches to study
bacterial amyloids by focusing on the well-studied curli amyloid
fibers expressed by Enterobacteriaceae. These methods exploit the
specific tinctorial and biophysical properties of amyloids. The
methods described here are straightforward and can be easily applied
by any modern molecular biology lab for the study of other bacterial
amyloids.
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