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Synergism of Staphylococcus aureus Colonization and Allergic Reaction in the Nasal Cavity in Mice.
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asfyso
May 8, 2012, 6:49:56 AM5/8/12
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Int Arch Allergy Immunol. 2012 May 3;159(1):33-40. [Epub ahead of
print]
Synergism of Staphylococcus aureus Colonization and Allergic Reaction
in the Nasal Cavity in Mice.
Hohchi N, Hashida K, Ohkubo JI, Wakasugi T, Mori T, Nguyen KH, Kuroda
E, Ikeno T, Taniguchi H, Suzuki H.
Abstract
Background: The aim of this study is to investigate the reciprocal
effect of Staphylococcus aureus colonization and allergic rhinitis in
an allergy model of mice. Methods: BALB/c mice with intraperitoneal
ovalbumin (OVA) sensitization and/or intranasal S. aureus inoculation
were prepared. The following 4 groups were designed: an OVA-sensitized
S. aureus-inoculated (AR-SA) group, an OVA-sensitized uninoculated
(AR) group, a nonsensitized S. aureus-inoculated (SA) group, and a
nonsensitized uninoculated (control) group. After intranasal OVA
challenge, nasal lavage fluid, peripheral blood, and nasal mucosa were
collected. Polymorphonuclear cells in the nasal lavage fluid were
counted, serum OVA-specific IgE and IgG1 were measured by enzyme
immunoassays, and IL-4, IL-5, and IFN-γ mRNAs in the nasal mucosa were
assessed by quantitative real-time reverse transcription-PCR. The
number of S. aureus in the nasal mucosa and lavage fluid was counted.
Results: Both eosinophil and neutrophil counts were larger in the AR-
SA group than in the other groups. Both IgE and IgG1 levels were
higher in the AR and AR-SA groups than in the SA and control groups,
and the IgG1 level was higher in the AR-SA group than in the AR group.
The expression of IL-4 mRNA was higher in the AR-SA group than in the
other groups, and the expression of IL-5 mRNA was higher in the AR-SA
group than in the SA group. The AR-SA group showed higher counts of S.
aureus in the nasal mucosa than the SA group. Conclusion: These
results indicate the mutually potentiating effect of S. aureus
colonization and allergic rhinitis.
print]
Synergism of Staphylococcus aureus Colonization and Allergic Reaction
in the Nasal Cavity in Mice.
Hohchi N, Hashida K, Ohkubo JI, Wakasugi T, Mori T, Nguyen KH, Kuroda
E, Ikeno T, Taniguchi H, Suzuki H.
Abstract
Background: The aim of this study is to investigate the reciprocal
effect of Staphylococcus aureus colonization and allergic rhinitis in
an allergy model of mice. Methods: BALB/c mice with intraperitoneal
ovalbumin (OVA) sensitization and/or intranasal S. aureus inoculation
were prepared. The following 4 groups were designed: an OVA-sensitized
S. aureus-inoculated (AR-SA) group, an OVA-sensitized uninoculated
(AR) group, a nonsensitized S. aureus-inoculated (SA) group, and a
nonsensitized uninoculated (control) group. After intranasal OVA
challenge, nasal lavage fluid, peripheral blood, and nasal mucosa were
collected. Polymorphonuclear cells in the nasal lavage fluid were
counted, serum OVA-specific IgE and IgG1 were measured by enzyme
immunoassays, and IL-4, IL-5, and IFN-γ mRNAs in the nasal mucosa were
assessed by quantitative real-time reverse transcription-PCR. The
number of S. aureus in the nasal mucosa and lavage fluid was counted.
Results: Both eosinophil and neutrophil counts were larger in the AR-
SA group than in the other groups. Both IgE and IgG1 levels were
higher in the AR and AR-SA groups than in the SA and control groups,
and the IgG1 level was higher in the AR-SA group than in the AR group.
The expression of IL-4 mRNA was higher in the AR-SA group than in the
other groups, and the expression of IL-5 mRNA was higher in the AR-SA
group than in the SA group. The AR-SA group showed higher counts of S.
aureus in the nasal mucosa than the SA group. Conclusion: These
results indicate the mutually potentiating effect of S. aureus
colonization and allergic rhinitis.
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