MED:Clustered Water (2) (was:MED: RES: Debunking Clust...)

[Denise Larin]

Here is part 2 of the information concerning clustered water (Aqua Resonance)
If I can be of any help in answerin your questions please feel free to ask.

Take care,

Denise Larin
dla...@accent.net

Aqua Resonance. (Clustered Water)
A summary of the work of Dr. Lee Lorenzen
By Stephen Cherniske,M.S.
(THE NUMBERS REFER TO NOTES AT THE END)

Background
Aqua Resonance is the trade name given to clustered water solutions developed
by Dr. Lee Lorenzen. To realize the value and potential of these solutions,
it is useful to review some organic chemistry so that we understand the
framework upon which this breakthrough research has been conducted.

The water molecule is formed by the covalent bonding of two hydrogen atoms to
one oxygen atom. To complete its outer shell, oxygen needs two electrons, and
it obtains these by sharing an electron with each of two hydrogen atoms. These
bond to the oxygen atom to form a triangular structure, and this shape is
important because it forms the basis for many of the solutions and compounds
that support life.

Now, these covalent bonds are polar bonds, that is the shared electrons are
attracted more strongly to (or "spend more time with") the oxygen nucleus than
to the hydrogen nuclei. This creates a small negative charge in the area of
the water molecule near the oxygen nucleus, and a small positive charge near
the two hydrogen nuclei. Clusters form because the positive charge of the
hydrogen atoms of one water molecule is attracted to the negative charge of
the oxygen of another water molecule. It is well known that such hydrogen
bonds play important roles in many biological compounds, and are essential for
maintaining the shape of large molecules such as proteins and nucleic acids.
As we will explain, scientists are now discovering something rather remarkable
and revolutionary, and that is that a molecule's shape is as important to its
function as its composition.

Cluster technology has opened an entirely new arena of materials science.
Iron, for example, adsorbs hydrogen 1,000 times as fast in ten-atom clusters
as it does in 17-atom clusters. Electronic bonding patterns, determined by
cluster shape, alter the behavior and properties of solids, liquids and even
gasses. When electrons are shared by the whole cluster in a delocalized
pattern, negative charge is evenly distributed and the cluster may take on
certain aspects of solid metal, such as conductivity. When the electrons are
all tightly bound to atoms, the clusters resemble discrete molecules. The
discovery that small changes in cluster size can produce large differences in
behavior strengthens the notion that clusters represent a distinct phase of
matter.

If this is all new to you, you are not alone. The emerging science of
structural biology is shaking up what most of us understood were immutable
laws. You can, for example, take a protein from the body that has a well
documented function. Replace many of the amino acids, but maintain the same
exact structure, and that protein will function in the same way. We learn how
water transports protein through the body, but are now finding out that the
movement of water is just as precise. In fact, cell membranes throughout the
body utilize special proteins to transport water (1), and intracellular water
turns out to be highly organized. Instead of the amorphous solute that we all
saw in Bio 101, water appears to exist within the cell in a complex
multilayered structure.

Molecular biologist Gilbert Ling, using X-ray diffraction and Neutron
scattering techniques, has shown that cytoplasmic water is remarkably
different from a simple dilute aqueous solution (2). Roschach at Rice
University and Clegg at the University of California have both confirmed this
using quasi elastic neutron scattering (QUENS)(3). Srivastava and Bernhard
have shattered the idea that biochemical reactions take place in the cell by
free diffusion. Their investigation of the glycolic pathway illustrates that
metabolic intermediates are virtually all enzyme-bound and are <channelled>
between enzymes through structured water (4).

Now, one of water's most important functions is to maintain and influence
protein structure. Michael Rodbell won a Nobel prize for his work with protein
folding and G peptide, specifically for elucidating the role that these
proteins plays in cell communication or signal transduction. In and through
this protein, there is a matrix of clustered or organized water (5)(6). In
fact, Dr.Julia Goodfellow at the Department of Crystallography in Birbeck
College, London, has shown that it is the interaction of structured water with
biological macromolecules that causes the protein folding. And so begins our
discussion of biophysics.

Water, water everywhere.
The water within our body, known as <biowater>, is <intimately involved> in
cell physiology; not just in the movement of nutrients and the removal of
toxins and waste. It is becoming clear that water plays an active role in cell
communication and thus literally thousands of metabolic functions.

I was taking graduate courses in physiology in the mid 70's, and remember
quite clearly that cell communication was limited to a discussion of receptor
sites, hormones, and the intricate but fairly well-understood workings of the
brain and nervous system. What no one seemed to want to tackle was the
enormous question of how the <rest> of the body communicated. Obviously,
trillions of cells contribute to the miraculous organism called man, and these
cells, contrary to the diagrams in the textbooks, were not isolated little
bags of cytoplasm stacked together into human form. There was inherent in this
study, an enormous missing puzzle piece having to do with the interactions of
cells apart from the mechanical and neurologic communication systems.

The transmission of information from DNA to RNA is a good case in point. We
had beautifully stained microscopic views of mitosis. It was easy to
understand the breaking of nucleotide bonds and the replication of the double
helix. But cells perform thousands of other functions which are all
controlled by DNA. I was perplexed by drawings of DNA fragments "breaking off"
and attaching to RNA. "This is impossible," I protested, and my professor
agreed. "It's just the way they choose to represent a process that is not
understood," he replied.

Well, we now know that the flow of information from DNA is constant and that
the schematic textbook representations were utter nonsense. We now know that
in the core of that double helix is a column of water clusters, and that
information is transmitted at lightning speed via resonant frequencies. That's
right, vibrations.

I can hear the gnashing of teeth. You hate that right? It sounds too
imprecise. But the scientific support for this scenario is undeniable,
involving principles of physics as much as chemistry. It may sound weird to
hear biochemists talking about semi-conduction, electrical amplification and
transduction, but that is the new frontier, and the implications of this
research for clinical medicine are astounding.

It has been demonstrated that cells possess individual and cooperative
resonant patterns that change with age and metabolic efficiency. After
thirteen years of painstaking work and more than 12,000 case histories, Dr.
Lorenzen has been able to show that these resonant patterns can be enhanced,
producing beneficial effects on tissue and organ homeostasis. Here is the
story in brief.

Early Work with Clustered Water Solutions
Franks and coworkers performed statistical and mechanical analysis of water
and referred to metabolically active water as a <simultaneous organization of
molecular states> (7). Dr.Martin Armbruster of the Physics University in
Freiburg showed that negatively charged water clusters were extremely common
in human physiology, being composed of eight or more negatively charged water
molecules (8). At the McLennon Physics Research Laboratory, University of
Toronto, Dr.L.M.Banic found at least 49 unique molecular constructions of
water molecules surrounding SO2, CO2 and charged ions (9). Similar complex
structures have been described by Brodskaga (10), Curtiss (11), Egelstaff
(12), Fowler (13) and Lee (14).

Inquiry into the role of organized water in cell systems expanded rapidly. The
action of trypsin was finally explained. Remember that trypsinogen is produced
by the pancreas, acted upon by enterokinase, and converted to active trypsin
in the small intestine. The trypsin the activates other proteolytic enzymes
(chymotrypsinogen to chymotrypsin, and procarboxypeptidase to
carboxypeptidase). Much of this activity could not be explained by the
mechanics of simple enzymes. Instead, it appears to be signal transduction
through clustered water. Structural biochemists have learned that the trypsin
molecule contains 300 water sites, most of the clustered into hydrogen bonded
networks, and arranged in spheres like layers of an onion. The authors of one
recent study observed that:
"When the effects on surface accessibility by neighboring molecules in the
crystal lattice are taken into consideration, only about 29% of the trypsin
surface does not interface ordered water. About 25% of the ordered water is
found in the second hydration sphere. In many instances theses waters bridge
larger clusters of primary layer waters. It is apparent that, in certain
regions of the crystal, the organization of ordered water reflects the
characteristics of the crystal environment more than those of trypsin's
surface alone (15)."

New data is shattering our notions of cell structure, illustrating a
"microheterogeneity" of cytoplasmic space, including a structural organization
of proteins and organized water that may extend through literally thousands of
cells. One group of researchers developing a new model of cellular respiration
observed:
"All the numerous data show convincingly that cellular metabolism cannot be
understood if cell interior is considered as a homogeneous solution, and it is
necessary to use the theories of organized metabolic systems and
substrate-product channelling in multienzyme systems to understands metabolic
regulation of respiration (16).

Biochemists, in fact, are finding that cluster formations abounds in a variety
of tissues, and are using the term 'metabolon> to refer to a metabolically
active cluster composed of enzymes and organized water (17). Indeed, Beekmans
and co-workers have stated that "large amounts of water are believed to be
organized in layers at the surface of intracellular structural proteins and
membranes (18).

The plot thickens
In 1992, research was presented to elucidate the role that organized or
clustered water plays in the aging process. Japanese investigators using
magnetic resonance imaging found that aging results not only in dehydration,
but that the water that remains in the tissues undergoes significant
structural changes (10). The predominate morphological change is an increase
in biowater bound to biological macromolecules and a concomitant decrease in
"free" clusters. The dynamic activities of biowater, such as cell
communication, molecular movements, nutrient delivery, detox and diffusion all
decline with age.

Until recently, it was assumed that this decline in metabolic efficiency was
simply an inevitable result of aging. But Lee Lorenzen challenged that
assumption by producing clustered water and stabilizing it so that it can be
consumed. The discovery was an accident; the result of cold temperature
sterilization research in which liquids were exposed to powerful magnetic
pulses. Lorenzen found that this treatment produced exposed rings and
ultimately clusters. Most important, he was able to alter the cluster
formations to reproducible units and stabilize them with ceramic technology.

Through careful experimentation, Lorenzen has produced clusters that closely
resemble the ordered water that is metabolically active in human systems.
These clusters can be seen under 20,000 X magnification, and they have been
stabilized for up to two years. Thus we can now increase the amount of
clustered water in our body. But the story does not end here.

It has been found that water clusters vibrate at specific resonant
frequencies, and these frequencies can help restore homeostasis to cell
structures in the body through signal transduction (20). Transduction is the
process by which one form of energy is converted to another. We all experience
this daily in the operation of our senses. Our eyes transduce of convert light
energy into electronic signals which are sensed and recorded in the back of
the brain. our ears are low-frequency sound transducers. Smell receptors are
chemical transducers.

When clustered water is consumed, high frequency information is transmitted to
proteins in the mouth, esophagus and gastrointestinal tract. These proteins
amplify the signal and send it in a cascading wave to other connected cells.
This wave of information is carried throughout the body like a "wake-up call"
to restore normal function.

As a health-care modality, this represents a remarkable opportunity to
stimulate selfhealing. Dr. Franco Bistolfi has described these dynamics in
what he terms the Bioelectronic (or bioconductive) Connectional System of BCS
(21). The components of this system include the cell's protein cytoskeleton
and extracellular proteins such as collagen fibers and keratin filaments.
Together with ordered water, Bistolfi proposes that these structures "are the
morphological expressions of a large and unitary cooperative system for
coherent communications among cells, by means of piezoelectric interactions
and photon/phonon transduction of electromagnetic signals, of both endogenous
and exogenous origin (22).

Given the extremely important role played by inter and intracellular water,
some have even suggested that the increased morbidity and mortality associated
with obesity may in part stem from this factor. Total body water is related
primarily to the fat content and age of the body. An obese man will have a
water content approximately 45% while a lean man weighting the same, will have
a water content of 70% or more. The water content of an 'average" 45 year old
man is 63%, but by age 70, this decreases to approximately 47% (23). Clearly,
the ability to restore optimum levels of ordered water to this system will
have profound effects on the entire body. But there's even more.

The fundamentals of cellular resonance
Resonance is a phenomena that occurs throughout nature. At the atomic level,
we know that electrons whirl about the nucleus in precise energetically
defined orbits. In order to move an electron from a lower to a higher orbit,
a quantum of energy with very specific frequency characteristics is required.
In fact, an electron will only accept energy of the appropriate frequency, and
if the electron falls to a lower orbit, it will release energy of that very
same frequency. This specific quantum of energy is known as the resonant
frequency.

The phenomena of resonance is the principle behind MRI scanning. Atoms and
molecules have individual resonant frequencies that will only be excited by
energies of precise vibratory characteristics. Mamerman, in fact, has shown
that the absorption spectra for DNA is 42 octaves above the C tone (C = 256
Hz) (24). It is well accepted that the wavelengths maximally absorbed by cell
systems are equivalent to those spectra emitted when the substance is excited.
With the correct chemical and or electromagnetic stimulation, healthy tissue
systems will respond with predictable resonant emission frequencies. Medical
researchers, most notably in Germany and Japan, are now experimenting with
therapeutic MRI devices. This represents a new paradigm in preventive health
care; the normalization of subtle but now well-defined resonant frequencies in
cell systems. And while it may be difficult to accept, (the obvious
description being "fine-tuning") this reality is nevertheless upon us.

MRI technology has revealed that aberrant enzymatic or metabolic activity will
affect the primary tissue resonant frequency (evidenced by changes in T1 and
T2 relaxation times) and create an emission phase shift phenomenon. Current
models of disease management initiate treatment only after the development of
symptoms. But those symptoms are the results of gross biochemical imbalance
induced by many factors. In chronic degenerative disease, cellular imbalance
is usually asymptomatic until the disease has reached an advanced stage of
development. Preceding this stage, disruptions in cell water multilayers will
have already produced aberrant coherent information transfer that can be
measured. Using a device known as a Magnetic Resonance Spectral Analyser (MRA)
and in cooperation with pathologist Hoang Van Duc at the University of
Southern California, prepathological conditions have been predicted, detected
and later confirmed based upon aberrant shifts in tissue resonance. Even more
exciting, of course, is the possibility of correcting those aberrant
frequencies using non-invasive, safe and inexpensive means.

Lorenzen's work with clustered water solutions with precise dimensions and
resonant frequencies is the crest of that wave. We know now that structured
water within the cell acts as a transducer of chemical and bioelectric energy
(25). The resonant frequency produced by the transduction organizes nucleic
acids and proteins, providing a unified system for cell repair and
replication. And while it is well understood that this unified system breaks
down with age, Dr.Lorenzen was among the first to assert that the resulting
decrease in metabolic efficiency is both an effect and a cause of the aging
process. Recent work by Haussinger and co-workers shows that minute changes in
cell hydration procedure dramatic alterations in cellular metabolism and gene
expression (26). Lorenzen has shown that metabolic efficiency can be enhanced
by restoring tissue levels of clustered water, and furthermore, that this
water can impart beneficial effects throughout the body via signal
transduction (27) (28). His patent-pending Nanocluster-Template Induction
Process. has resulted in production of a number of solutions with clinically
proven therapeutic value.

Many of these solutions have been used in Japan for nearly a decade, with more
than 12,000 case histories recorded. They have been administrated to
in-patients at the Kyowa Hospital in Kobe, Japan with the following results
reported by Dr.H.Hayashi:
1. Declines in blood sugar levels in diabetics patients
2. Improvements in peripheral circulation in diabetic gangrene
3. Declines in uric acid levels in patients with gout
4. Improvements in liver function in hepatic disorders
5. Improved healing of gastroduodenal ulcers and prevention of reoccurrence
6. Improvement in hypertension and hypotension
7. Improvements in asthma, urticaria, rhinitis, and atopic dermatitis
8. Improvement in post gastrectomy diarrhea
9. Improvement in postoperative bowel paralysis
10. Improvements in serum bilirubin levels in newborn infants.

Aqua Resonance AM, Aqua Resonance PM and Aqualogics Eye Lotion are now
available in U.S. These solutions are produced in licensed pharmaceutical
laboratories under quality control conditions that meet or exceed all FDA and
GMP guidelines. Valuable clinical experience has been gathered by a number of
American physicians including Bruce O'Dell,M.D, Kenneth J.Frank, M.D., F.Pearl
McBroom, M.D., Gene Denk, M.D., Gerald H.Ross, M.D., and Chunhye Kim Lee,
Ph.D. While an extensive list of therapeutic benefits could be made, there are
many who will discount any evidence other than doubled blind
placebo-controlled studies. I would like to suggest that while we are waiting
for this level of research, action can and should be taken based upon the
compelling data that we now have. After all, a year long assessment by the
Office of Technology Assessment has found that only 10-20% of all procedures
used in medical practice have been shown to be of benefit by controlled
clinical trials (29).

The primary consideration for most of us is safety, and the oral
administration of 100% pure triple distilled water is highly unlikely to cause
harm. The potential benefits, on the other hand, are remarkably diverse. If
you would like to speak with one of the above physicians, I invite to contact
Dr.Kenneth Frank at (805) 730-7420. "The greatest impediment to scientific
innovation," wrote Harvard paleontologist Stephen Jay Gould, "is usually a
conceptual lock, not a factual lack".

SCIENTIFIC REFERENCES:
The first reference that I would like to provide is the work of Dr Lee
Lorenzen. BTW, Dr Lorenzen's wife suffers from CFS and FMS and this has been,
I think, a great motivation for him in his researches.

Dr. Lee Lorenzen is the inventor and developer of a variety of nutritional
products created with resonant cluster technology. He is the discoverer of
the cluster template induction process.

Dr. Lorenzen has a Ph.D. in nutritional biochemistry and is licensed in
clinical nutrition with the American Licensing Board of Nutrition. In
February 1991, he became a founding member and Vice Chairman of the Japanese
American Resonance Research Society, Tokyo, Japan.

Dr. Lorenzen is a member of the German American Society for the Advancement
of Biological and Oxygen Therapies, Bad Fussing, Germany.

He has written and narrated twelve 30-minute videos on nutrition and
prevention for the general public and has lectured extensively on the life
sciences in 42 states and 25 countries. He is a sought-after speaker on
stage, video and television.

Dr. Lorenzen has published articles on resonant field theory and the Russian
culture in Proceedings of the Western Pharmacology Society, Life Sciences
(London), Food Technology Japan, Up-to-Date Food Processing, and La Vie
Japan.

Dr. Lorenzen's work is widely known in Asia and he is widely recognized in
Europe and around the world for his work in cluster resonant water
technology.

He was recently honored at the University of Paris and also the University
of Oslo, Norway. In Norway, he was awarded an Honorary Doctorate Degree in
Medicine for developing alternative therapies in the healing arts.

Dr. Lorenzen received his M.A. in biology from California State University,
Fullerton and his Doctorate in nutritional biochemistry at the Metropolitan
Collegiate Institute, London, England.

Articles written by Dr. Lorenzen include:

Navarro, G. and L. Lorenzen (1976). "Effects of parachlorophenylalanine on
brain amines and drug-induced sleep." Proceedings of the Western
Pharmacology Society. June 1976.

Fairhurst, A. and L. Lorenzen (1977). Use of 20-25 diazacholesterol as a
model of myotonia dystrophica in the rat." Life Sciences (London)

Lorenzen, L. (1989). "The resonant field theory --- what is resonant water?"
Food Technology Japan v.32 n7:69-78.

Lorenzen, L. (1989). "ROD Water Information." Up-to-Date Food Processing
(Japan) v.24, #7. July 1989.

Lorenzen, L. (1989). "Lacto-Bio Culture: antiviral activity in Epstein-Barr
Syndrome." La Vie Japan v.9, September 1989.

Other references: I have personnaly read most of these articles.

1. Farinas J; Van Hoek AN; Shi LB; Erickson C; Verkman AS. Nonpolar
environment of tryptophans in erythrocyte water channel CHIP28 determined by
fluorescence quenching. Biochemistry, 1993 Nov 9, 32(44):11857-64.

2. Ling GN. Solute exclusion by polymer and protein-dominated water;
correlation with results of nuclear magnetic resonance(NMR) and calorimetric
studies and their significance for the understanding of the physical state of
water in living cells. Scanning Microscopy 1988; 2(2):871-884.

3. Clegg JS. On the physical properties and potential roles of intracellular
water. In: Organization of Cell Metabolism. GR Welsh and JS Clegg(eds.) 1987
Plenum Press, New York.

4. Srivastava DK, Bernhard SA. Metabolite transfer via enzyme-enzyme
interactions. Science 1986;234:1081-84.

5. Rodbell M.The role of GTP-binding proteins in signal transduction from the
sublimely simple to the conceptually complex. Current Topics in Cellular
Regulation, 1992,32:1-47.

6. Waterson JG. A model linking water and protein structures. Biosystems
1988;22(1):51-4.

7. F Franks, et al. Biophysics of Water. 1982. Wiley.,New York.

8. Ambruster M. Negatively charged water clusters. Phys Rev Lett
1981;47(5):323-6.

9. Banic LM. Equilibrium constants for clustering of neutral molecules about
gasseous ions. J Chem Phys 1981; 83(12):6432-48.

10. Brodskaga EN. Molecular dynamics investigation of the structure of water
microclusters. Leningrad U.Kolloidin.Zh. 1986; 48(1): 3-16.

11. Curtiss LA. Thermodynamic parameters of the dimer and evidence for higher
polymers in water vapor. Chemical Engineering Division, Argone National
Laboraories, Illinois.

12. Egelstaff PA. Structural changes in low temperaturewater. Chem Phys 1986;
101(1):175-81.

13. Fowler PW. The effects of rotation, vibration and isotopic substitution on
the electric dipole moment, magnetizability, and the nuclear magnetic
shielding of the water molecule. Department of Chemistry, University of
Sheffield. Mol Phys 1981 43(1):65-82.

14. Lee WK. A molecular dynamic study of water microclusters surrounding a
phosphate ion. J Chem Phys 1981; 75(6):3040.

15. Kossiakoff AA;Sintchak MD;Shpungin J;Presta LG. Analysis of solvent
structure in proteins using neutron D2O-H2O solvent maps: pattern of primary
and secondary hydration of trypsin. Proteins, 1992 Mar, 12(3):223-36.

16. Saks VA;Khuchua ZA;Vasilyeva EV;Belikova OYu;Kuznetsov AV. Metabolic
compartmentation and substrate channelling in muscle cells. Role of coupled
creatine kinases in in vivo regulation of cellular respiration--a synthesis.
Molecular and Cellular Biochemistry, 1994 Apr-May, 133-134:155-92.

17. Beeckmans S;Khan AS;Van Driessche E;Kanarek L. A specific association
between the glyoxylicacid-cycle enzymes isocitrate lysase and malate synthase.
European Journal of Biochemistry, 1994 Aug 15,224(1):197-201.

18. Beeckmans S;Khan AS;Van Driessche E;Kanarek L. Immobilized enzymes as
tools for the demonstration of metabolon formation. A short overview. Journal
of Molecular Recognition, 1993 Dec, 6(4):195-204.

19. Katayama S. Aging mechanism associated with a function of biowater.
Physiol Chem Phys & Med NMR. 1992; 24:43-50.

20. Bistolfi F. A hydrogen-harps model for intracellular communication and its
implications for the second genetic code. Panminerva Medica, 1990 Jan-Mar,
32(1):4-9.

21. Bistolfi F. The bioconductive connectional system: radiobiologic and
therapeutic implications. Radiologia Medica, 1990 Sep, 80(3):203-6.

22. Bistolfi F. The bioelectronic connectional system (BCS): a therapeutic
target for non ionizing radiation. Panminerva Medica, 1990 Jan-Mar,
32(1):10-8.

23. Goldberger E. A Primer of Water, Electrolyte and Acid-Base Syndromes.
1970, Lea & Febiger, Philadelphia.

24. Frohlich H. Coherent Excitations in Active Biological Systems.
Springer-Verlag, Berlin 1985.

25. Haussinger D;Lang F;Gerok W. Regulation of cell function by the cellular
hydration state. American Journal of Physiology, 1994 Sep, 267(3 Pt
1):E343-55.

26. Lorenzen L. The resonant field theory - what is resonant water? Food
Technology Japan, 1989;April.

27. Lorenzen L. Lacto-bio culture:antiviral activity in Epstein-Barr Syndrome.
Le Vie Japan 1989;9:247.

28. Assessing the Efficacity and Safety of Medical Technologies. U.S.
Government Printing Office, 1978.

Take care,

Denise Larin
dla...@accent.net

--