Jesus Christ.
Interesting question! That's an easy one since I'm a DOS
game programmer. I prefer MS-DOS 6.22 since I have the
official software for it. However, I don't have the
original hardware. It is being VirtualBox. The closest to
that is DOSBox 0.74 since it emulates MS-DOS 6.22 in
addition to being able to use a Microsoft XBOX360 controller
and SONY PS One Controller (both needing a USB adapter for
the USB port on the PC). Also, DOSBox 0.74 is able to
reproduce sound that I can control where as MS-DOS 6.22
needs soundblaster, which I'm not used to right now.
However, I do like Microsoft Windows XP for a third
alternative.
Cheers from a heavy coffee drinker.