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[Priamo Gregory]

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Objective:To build a digital cross-sectional images date set of human normal larynx with high-precision.Method:A fresh frozen normal human larynx was embedded using Kawamot's Film Method and then serially sectioned at 10 μm with a LeicaCM3600XP Cryomacrotome, meanwhile digital images of the block surface were recorded after every section with a Nikon D750 camera and stored respectively as JPG and RAW files. All images were cropped to display a constant area on the block surface by photoshop7 and stored as TIFF files. Result:A total of 6 656 images were recorded in 24-bit RGB resolution, the complete data file is 97.5 GB and 174 GB. After elimination of image size variation, all images were re-sampled at a final resolution of 40 μm/pixel and the complete data file is 107 GB. Conclusion:It's feasible to use the combination of Kawamot's Film Method,Digital Single Lens Reflex with high-resolution and regular block face-based alignment to build high-precision digital images date set, which could apply to some partial organs that resemble larynx.

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