AlliGator 0.89 release

[AlliGator Software Support]

Installer available at: https://github.com/smXplorer/AlliGator/blob/main/installer/latest/AlliGator 0.89 Installer.zip (use the button on the left hand side of the window if the file is not automatically downloaded).

• Bug Fixes

  • Fixed occasionally erroneous Phasor Graph references-connecting segment (and limited it to the actual segment)

  • Fixed bug in Remove Phasor from Plot in Phasor Graph

  • Fixed “Save Phasor Calibration (Single/Map/Series)” dialog not opening the last used folder

  • Fixed discrepancy between calibration phasor frequency and current frequency not being flagged as a problem

  • Fixed some lagging image contrast update when moving the image histogram cursors

  • Fixed broken installation process (when checking whether a new version is available online)

  • Fixed formatting of some complex string outputs

  • Fixed a bug where the “Rectangle Image Profile” calculation was performed even when the Image Profile Window was not open, slowing down AlliGator when a full-frame ROI or any large rectangle ROI was drawn

• New Features

  • Added option to use background-subtracted intensity in Image Profile Window (Menu->Image Intensity->Background-subtracted). The background value subtracted (per pixel) is that defined in Settings>>Fluorescence Decay>>Decay Pre-Processing>>Constant Background

  • Added option to use the Current Frequency or one of its harmonics in the “Single-Exponential Phasor Locus (SEPL)>>Phasor” Tab of the Settings window

  • Now eliminates NaN phasors from Parameter 2 vs Parameter 1 computation in Phasor Graph

  • Added Fit Termination criteria output to the Notebook

  • Added Residual Sum of Squares (RSS), Akaike Information Criterion (AIC), Bayesian Information Criterion (BIC) to Notebook output

  • Implemented MLE fit based on Ted Laurence’s modification of the Levenberg-Marquardt algorithm

  • Added Fit Method Benchmark Tool to Analysis>>Tools menu

Reminder:

There is not need for Administrator rights to install AlliGator, as it is installed on a per user basis in the local user application folder.

AlliGator version 0.51 and above use LabVIEW 2021 SP1 runtime (free): https://www.ni.com/en-us/support/downloads/software-products/download.labview-runtime.html#443841

and Vision Development Module 2021 runtime (license required): https://www.ni.com/en-us/support/downloads/software-products/download.vision-development-module-runtime.html#409837