A doubt in Hanif's paper

[Deepu Mathew]

Dear Hanif

While reading your paper "Sivalingam, P.N., Singh, D., Changal, H.K., Khan, H., Sangwan, R., Samadia, D.K. and Sharma, S.K., 2012. Development of PCR-based diagnostic probe to detect begomoviruses infecting chilli in the hot arid region of Rajasthan. Archives of phytopathology and plant protection, 45(3), pp.301-309." I got a doubt.

In the PCR, you have used 0.5microgrammes of DNA whereas, usually, we use up to 50 nanogrammes only in a usual PCR. Why we should use nearly 100 times more DNA? One reason may be that the proportion of viral DNA will be very less when we extract viral DNA directly from infected samples. Even with that, I think only picogrammes of DNA will be sufficient to get the locus amplification in PCR. In the paper, you have tried DNA concentrations starting from 0.50 microgram, then 1.00 microgram etc. Have you attempted with normal concentrations such as 10-30 microgram?

Regards

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Deepu Mathew, Ph. D. |

Assistant Professor |

Centre for Plant Biotechnology and Molecular Biology |

Kerala Agricultural University | KAU Post, Thrissur |

Kerala - 680 656 | INDIA |

Phone: +919446478503 |

On 25 June 2017 at 14:30, Hanif Khan <hanif...@gmail.com> wrote:

Thank you Dr Zahoor.

May the choicest
blessing of Allah
fill your life with
joy and prosperity.
EID MUBARAK

On 25-Jun-2017 1:35 PM, "Zahoor Dar" <zaho...@gmail.com> wrote:

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